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Rudas VA, Piven’ NM, Rivkin MI, Vershinin AV, Infante DC

The genetic transformation of Lycopersicon peruvianum var. dentatum by using Agrobacterium tumefaciens carrying a plasmid with the human beta-interferon gene

 

TSitologiia i genetika 1997, v 31, Issue 2, 17-22


Wild tomato plants of Lycopersicon peruvianum var. dentatum were transformed with pG11 plasmid having human beta-interferon (hIFN-beta) and NPTII genes. Transformation was demonstrated by polymerase chain reaction (PCR) and ELISA assay. Expression of hIFN-beta gene was identified by Western blot analysis. Transgenic plants produced seeds and F1 generation inherited integrated traits.

Rudas VA, Piven’ NM, Rivkin MI, Vershinin AV, Infante DC The genetic transformation of Lycopersicon peruvianum var. dentatum by using Agrobacterium tumefaciens carrying a plasmid with the human beta-interferon gene // Tsitol Genet. – 1997. – 31(2). – P. 17-22.



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