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Rymar’ S.E., Isakova I.A., Kirilenko S.D., Kordium V.A.
The construction and use of inosine-containing primers for searching for and identifying the genes of insecticidal crystal proteins in Bacillus thuringiensis
TSitologiia i genetika 1999, v 33, Issue 2, 74-78
The specific to 3 types of Cry genes primers containing inosine were constructed to find crystal insecticidal protein Bacillus thuringiensis genes using PCR. A number of new B. thuringiensis strains isolated in Ukraine were investigated using these PCR primers. As a results, Cry genes were found, some of them were identified and demonstrated high homology to Cry1Ba2 and Cry1Bc genes.
Rymar’ S.E., Isakova I.A., Kirilenko S.D., Kordium V.A. The construction and use of inosine-containing primers for searching for and identifying the genes of insecticidal crystal proteins in Bacillus thuringiensis // Tsitol Genet. – 1999. – 33(2). – P. 74-78.
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