Main page
Contacts
Contents
Themes
Subscription
Information to authors
Editorial board
In Russian
|  |
Berezovskaya O.P., Sidorenko A.P., Mukha D.V.
Structural Characteristics of Osteopontin mRNA.
Cytology and Genetics 2001, v 35, number 3, 35-42
Conservatism of primary and secondary structure of osteopontin mRNA in vertebrates of various taxes was studied using computer analysis and blot-hybridization procedure. Relatively high rate of evolution of a nucleotide sequence of osteopontin gene was revealed. Formation of hairpin-loop structures by adjacent sequences and a high content of AU-pairs indicate more flexible structure of the mammalian osteopontin mRNA as compared with the avian one. It has been detected some AU-enriched regions in 3´-terminal untranslated sequences, which presence is characteristic for short-lived mRNA. Evolutionary conserved subsequences in 3´- and 5´-terminal regions were suggested to be determinants of RNA-protein interaction in cytoplasm.
|
