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Investigation of Molecular-Genetical Polymorphism in Three Cattle Breeds

Zubets M.V., Burkat V.P., Sivolap Yu.M., Kuznetsov V.E., Lovenchuk I.N.

 




Determination of cattle breed and assignment of individual animals to that or another group based on distinctions of genotype is of great importance in genetics and selection. Using of PCR-analysis opened new possibilities for typing DNA. The procedure of PCR was optimized for animal DNA. Two methods of PCR, RAPD and SSRP, were applied for investigation of molecular-genetical polymorphism in three cattle breeds (Ukrainian red-motley lactescent, Golshtine and Simmental). A dendrogram reflecting genetical mutual relations between analyzed animals was drawn. The level of homo-heterozygosity and the distribution of microsatellite alleles were determined. System for investigation of molecular-genetical polymorphism in the cattle breeds and characterization of genotype of individual animals were developed and tested on a real population.

Tsitologiya i Genetika 2001, vol. 35, no. 4, pp. 3-11



Zubets M.V., Burkat V.P., Sivolap Yu.M., Kuznetsov V.E., Lovenchuk I.N. Investigation of Molecular-Genetical Polymorphism in Three Cattle Breeds, Tsitol Genet., 2001, vol. 35, no. 4, pp. 3-11.




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