Barysheva I.A., Tulaeva M.I., Chisnikov V.S.

Study of the intracultivar variability of grape DNA using RFLP and PCR method

TSitologiia i genetika 2003, v 37, Issue 6, 31-38

Evaluation of DNA polymorphism among Vitis vinifera varieties using RFLP and PCR methods has been performed to choose a DNA technology for detection of grape intracultivar variation. DNA polymorphism of clones of the varieties Riparia Gluar, Riparia ? Rupestris 101-14, Cabernet Sauvignon, Riеstling reinskiy has been studied using Southern hybridization and amplification techniques. It has been shown that grape intracultivar variability of rDNA in Riparia ? Rupestris 101-14 and Cabernet Sauvignon clones was caused by the modification in AluI restriction sites of rDNA. DNA variability of the randomly amplified and inter-SSR sequences of the Riparia Gluar, Riparia ? Rupestris 101-14, Cabernet Sauvignon clones was also detected. A set of molecular DNA loci which can be used for grape clone identification has been obtained

Barysheva I.A., Tulaeva M.I., Chisnikov V.S. Study of the intracultivar variability of grape DNA using RFLP and PCR method // Tsitol Genet. – 2003. – 37(6). – P. 31-38.