Melnik V.M., Andreev I.O., Speredonova K.V., Kunakh V.A.

Restriction mapping and variability of 18S-25S ribosomal genes in some species of Gentiana genus

Cytology and Genetics 2003, v 37, number 5, 65–71

Mapping of rRNA genes in four species of Gentiana genus has been carried out by blot-hybridization method using some restriction endonucleases. The following characteristics of Gentiana rDNA structural organization have been revealed: 1) interspecific and intragenomic variability of the length of ribosomic repeats; 2) conservativity of the transcribed region; 3) variability of the length and location of Hind-III site in non-transcribed spacer; 4) interspecific variability of the number of copies. Comparative analysis of the constructed restriction maps of Gentiana species and some other plants revealed the similarity of restriction site location in the transcribed DNA region.