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Analysis of structural peculiarities of plant α-tubulins determining the increased cold tolerance

Nyporko A.U., Demchuk O.N., Blume Ya.B.

 


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The uniqueness of the point substitutions in the sequences of two α-tubulin isotypes from psychrophilic alga Chloromonas that can determine the increased cold tolerance of this alga was analyzed. The comparison of all known amino acid sequences of plant α-tubulins enabled to ascertain that only M268→V replacement is unique and may have a significant influence on spatial structure of plant α-tubulins. Modeling of molecular surfaces of α-tubulins from Chloromonas, Chalmydomonas reinhardtii and goose grass Eleusine indica showed that insertion of the amino acid replacement M268→V into the sequence of goose grace tubulin led to the likening of this protein surface to the surface of native α-tubulin from Chloromonas. Alteration of local hydrophobic properties of α-tubulin molecular surface in interdimeric contact zone as a result of the mentioned replacement was shown that may play important role in increasing the level of cold resistance of microtubules. The crucial role of amino acid residue in 268 position for forming the interdimeric contact surface of α-tubulin molecule was revealed. The assumption is made about the importance of replacements at this position for plant tolerance to abiotic factors of different nature (cold, herbicides).

Tsitologiya i Genetika 2003, vol. 37, no. 6, pp. 3-11



Nyporko A.U., Demchuk O.N., Blume Ya.B. Analysis of structural peculiarities of plant α-tubulins determining the increased cold tolerance, Tsitol Genet., 2003, vol. 37, no. 6, pp. 3-11.




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