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Analysis of nuclear and mitochondrial genomes of transplastomic Salpiglossis sinuata plants obtained by transfer of transformed plastids from N. tabacum (+S. sinuata) cybrid

Sytnik E.S., Parij A.F., Komarnitzki I.K., Gleba Yu.Yu., Kuchuk N.V.

 


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New model system of plastid transformation has been proposed using a wild representative of Solanaceae family S. sinuata. Earlier obtained cybrid plants N. tabacum (+S. sinuata) were used for transformation experiments by PEG treatment of protoplasts with aadA gene that confers resistance to spectinomycin. Transformed S. sinuata plastome was transferred from N. tabacum (+S. sinuata) cybrid to S. sinuata wild type plants by somatic hybridization. Molecular analysis of nuclear and mitochondrial DNA has been performed.

Tsitologiya i Genetika 2003, vol. 37, no. 5, pp. 3-8



Sytnik E.S., Parij A.F., Komarnitzki I.K., Gleba Yu.Yu., Kuchuk N.V. Analysis of nuclear and mitochondrial genomes of transplastomic Salpiglossis sinuata plants obtained by transfer of transformed plastids from N. tabacum (+S. sinuata) cybrid, Tsitol Genet., 2003, vol. 37, no. 5, pp. 3-8.




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