Ovcharenko O.A., Komarnitsky I.K., Cherep N.N., Gleba Yu.Yu., Kuchuk N.V.

Obtaining of intertribal Brassica juncea+Arabidopsis thaliana somatic hybrids and study of transgenic trait behaviour

TSitologiia i genetika 2004, v 38, Issue 3, 3-8

Intertribal somatic hybridization between wild type Brassica juncea (L.) Czern. & Coss. and transgenic A. thaliana L. has been carried out. Genome of A. thaliana plants contained heterologous transposable element Spm/dSpm, reporter GUS gene, selective genes for kanamycin- (npt II) and phosphinothricin (bar) resistance. Hybrid nature of obtained plants was confirmed with their morphology, GUS hystochemical assay, PCR-RFLP, RAPD and isozyme analyses.

It was determined that heterologous transposable element Spm/dSpm is able to functionate in hybrid plants. There was no complete elimination of A. thaliana genetic material in the hybrids and the transgenes were stably maintained.

Ovcharenko O.A., Komarnitsky I.K., Cherep N.N., Gleba Yu.Yu., Kuchuk N.V. Obtaining of intertribal Brassica juncea+Arabidopsis thaliana somatic hybrids and study of transgenic trait behaviour // Tsitol Genet. – 2004. – 38(3). – P. 3-8.