Tryapitzyna N.V., Glazko V.I.

Polymorphism of DNA fragments flancked by microsatellite loci (ISSR-PCR) in cattle reproduced under low dose irradiation conditions

Cytology and Genetics 2005, v 39, number 5, 41-50

Polymorphism of DNA fragments flancked by microsatellite loci (ISSR-PCR) in cattle reproduced under low dose irradiation conditions. In this study the family analysis was performed of amplicon heredity (ISSR-PCR markers) in ancestral F₀ generation and daughter’s F₁ and F₂ generations of Holstein cattle reproduced in alienation zone of Chernobyl accident for investigation of genetic structure changes and polymorphism peculiarities under low dose irradiation. Increasing of the heterozygosity сalculated (Hc) in F2 generation has been found that may be considered as response to ecological stress factor. No new amplicons have been revealed which could be evaluated as mutation events.