Kishchenko E.M., Komarnitskii I.K., Kuchuk N.V.

Production of transgenic sugarbeet plants (Beta vulgaris L.) using Agrobacterium rhizogenes

Cytology and Genetics 2005, v 39, number 1, 9-13

Normal phenotype sugarbeet plants transformed with Agrobacterium rhizogenes were produced using direct regeneration from explants withouth hairy root phase. Kanamycin resistant plants and Ri-roots carrying the genes of neomycin phosphotransferase II and b-glucuronidase have been obtained. Integration of transgenes into sugarbeet genome was confirmed with GUS-assay and PCR using primers for the introduced genes.