Demchuk O.N., Nyporko A.Yu., Blume Ya.B.

Construction of three-dimensional models of Arabidopsis thaliana FtsZ-proteins on basis of crystal structure of archaebacterial FtsZ-GDP complex

TSitologiia i genetika 2006, v 40, Issue 1, 10-20

Three-dimensional models of FtsZ-protein complexes with GDF from Arabidopsis thaliana L. localized in cytosol (Entrez database code NP190843) and in chloroplasts (Entrez database code AAA82068) were developed. Crystal structure of the FtsZ-GDP complex from archaea Methanococcus jannaschii (PDB-code 1FSZ) was used as a matrix. Secondary structures of computed models contain ten β-strands. A chloroplast form of FtsZ-protein has ten α-helices and four 3₁₀-helices, whereas cytosolic form of protein has nine and three structures correspondently and neither a0-helix before nucleotide-binding domain nor C-terminal 3₁₀-helix in secondary domain. The T2-loop of nucleotide-binding pocket of chloroplast form of FtsZ-ptotein in position 49 contains non-charged alanin residue instead of the charged one which is typical for cytosolic and bacterial forms of proteins. At low sequence homology of FtsZ-proteins (approximately 47 %) the developed models demonstrate high coincidence with matrix both in the structures of nucleotide-binding pocket and in the whole molecule. The models are completely suitable for further studies of possible sites of binding with dinitroaniline herbicides.

Demchuk O.N., Nyporko A.Yu., Blume Ya.B. Construction of three-dimensional models of Arabidopsis thaliana FtsZ-proteins on basis of crystal structure of archaebacterial FtsZ-GDP complex // Tsitol Genet. – 2006. – 40(1). – P. 10-20.