Yatsyshina A.P., Glazko T.T., Kovaleva O.A., Pidpala O.V., Lukash L.L.

The possible ways of the diploidization of the polyploid germinative stem cells in the mouse BALB/c line

Cytology and Genetics 2006, v 40, number 6, 44-49

Quantitative and qualitative chromosome rearrangements in the cell line G1 established from a genital ridge of the 12,5 dpc BALB/c mouse embryo were analysed. Cytogenetic analysis was performed on the 75th passage of in vitro cultivation. It has been shown that by this passage the cell population was heterogenous. It is suggested that such heterogeneity may be caused by realization of two simultaneous processes namely the cell polyploidization and their secondary diploidization. These processes were accompanied by some chromosome destructions, and the creation of small new acrocentric chromosomes and large aberrant chromosomes as well as Robertsonian translocations. The present study demonstrates in vitro karyotype evolution of the mouse cell line G1 including the increased instability of the chromosome apparatus.

Key words: chromosome rearrangement, polyploidization, diploidization, Robertsonian translocation, karyotypic evolution