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Sindarovska Y.R., Sheludko Y.V., Gerasymenko I.M., Bannikova M.A., Kuchuk N.V.
Purification of recombinant GFP produced by Agrobacterum-mediated transient expression in Nicotiana excelsior
Institute of cell biology and genetic engineering, Zabolotnogo str. 148, Kyiv 03680, Ukraine
TSitologiia i genetika 2008, v 42, Issue 2, 16-20
Green fluorescent protein (GFP) is commonly used as a reporter protein in a wide range of biological experiments. The efficient protocol of Agrobacterium-mediated transient expression in Nicotiana excelsior was applied for quick preparative production of recombinant GFP. The protein purification scheme has been developed and included ammonium sulfate precipitation and Q-sepharose anion-exchange chromatography. It results in obtaining of a fraction with about 85 % GFP homogeneity and the protein yield of about 75 %.
Key words: Nicotiana excelsior; Agrobacterium tumefaciens; transient expression; recombinant protein; GFP
Sindarovska Y.R., Sheludko Y.V., Gerasymenko I.M., Bannikova M.A., Kuchuk N.V. Purification of recombinant GFP produced by Agrobacterum-mediated transient expression in Nicotiana excelsior // Tsitol Genet. – 2008. – 42(2). – P. 16-20.
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