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Stable expression of promoterless bar gene in transgenic rape plants

Sakhno L.A., Gocheva E.A., Komarnitsky I.K., Kuchuk N.V.

 


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Phosphinothricin resistant plants of two rapeseed (Brassica napus L. var. oleifera DC.) spring industrial cultivars were obtained using Agrobacterium tumefaciens leaf disk transformation. Vector constructions contained the promoterless coding sequence of phosphinothricin acetyltransferase (bar) gene located between two inverted lox-sites (elements of Cre/lox recombination system of P1 phage) and selective neomycinphosphotransferase II gene (nptII). Integration of the alien genes was confirmed by the PCR analyses. Stable and linked inheritance of foreign genes in Т1 and Т2 progeny was shown.

Tsitologiya i Genetika 2008, vol. 42, no. 1, pp. 21-28

E-mail: iicb iicb.kiev.ua

Sakhno L.A., Gocheva E.A., Komarnitsky I.K., Kuchuk N.V. Stable expression of promoterless bar gene in transgenic rape plants, Tsitol Genet., 2008, vol. 42, no. 1, pp. 21-28.




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