Kovalyova V.A., Gout R.T.

Molecular cloning and characterization of Scotch pine defensin 2

TSitologiia i genetika 2008, v 42, Issue 6, 55-60

A full length coding sequence corresponding to Scotch pine defensin 2 (PsDef2) was achieved by a PCR approach using P. sylvestris сDNA library as a template. It has the potential to encode a protein of 83 amino acids in length. The first 33 amino acids correspond to the N-terminal signal peptide. The mature protein contains domain gamma-thionin and possesses conserved residues which are common to all plant defensins.

Kovalyova V.A., Gout R.T. Molecular cloning and characterization of Scotch pine defensin 2 // Tsitol Genet. – 2008. – 42(6). – P. 55-60.