Shakhbazau A.V., Yakovleva G.A., Rodkina I.A., Kartel N.A.

Pleiotropic effects of chitinase gene from Serratia plymuthica> in transgenic potato

TSitologiia i genetika 2008, v 42, Issue 2, 3-9

We estimate the influence of transgenic bacterial chitinase from soilborne Serratia plymuthica onto agronomical important traits of potato, such as productivity and non-specific resistance. Transgene has been delivered into potato variety Delfin via Agrobacterial transformation with pGreen0229 and pBI121-based vectors. Growth inhibition of chitin-containing pathogenic fungi was shown. However, over 40 % of transgenic lines demonstrated decreased non-specific resistance to late blight (down to 62 % compared to control genotype), as well as 40–60 % productivity drop.

Key words: chitinase, transgene, potato, transformation, pleiotropic effects

Shakhbazau A.V., Yakovleva G.A., Rodkina I.A., Kartel N.A. Pleiotropic effects of chitinase gene from Serratia plymuthica> in transgenic potato // Tsitol Genet. – 2008. – 42(2). – P. 3-9.