Matvieieva N.A., Vasylenko M.Y., Shahovsky A.I., Kuchuk N.V.

Agrobacterium-mediated transformation of lettuce (Lactuca sativa L.) with genes of bacterial antigenes from Mycobacterium tuberculosis

TSitologiia i genetika 2009, v 43, Issue 2, 27-32

Transgenic plants of lettuce Lactuca sativa L. cv. Eralash, Sniezinka, Rubinovoje kruzevo with genes coding synthesis of tuberculosis antigenes have been obtained by Agrobacterium-mediated transformation. Cotyledons of in vitro seedlings were used as the initial material for transformation with plasmids pCB063 (genes ESAT6, nptII) and pCB064 (genes ESAT6:Ag85B(-TMD), nptII). PCR-analysis has shown the presence both selective and target genes in all plants analyzed. At the same time, the RT-PCR has shown that both the presence and the absence of a transcription of gene ESAT6 at a stable transcription of a gene nptII is possible.

Matvieieva N.A., Vasylenko M.Y., Shahovsky A.I., Kuchuk N.V. Agrobacterium-mediated transformation of lettuce (Lactuca sativa L.) with genes of bacterial antigenes from Mycobacterium tuberculosis // Tsitol Genet. – 2009. – 43(2). – P. 27-32.