TSitologiya i Genetika 2011, vol. 45, no. 5, 11-16
Cytology and Genetics 2011, vol. 45, no. 5, 277-281, doi: https://www.doi.org/10.3103/S0095452711050082

Regeneration of transgenic plants from Cichorium intybus L. var. foliosum Hegi hairy roots

Matvieieva N.A., Kishchenko O.M., Shakhovsky A.M., Potrochov A.O., Kuchuk M.V.

Transgenic plants were regenerated from Cichorium intybus L. hairy roots transformed with genes of tuberculosis antigenes ESAT6 and Ag85B or human interferon a2b. The plant regeneration was light-dependent and occurred on the media without growth regulators. The DNA PCR and RT-PCR analyses have shown the presence and expression both selective and target genes in all root lines and regenerated plants.

Keywords: Cichorium intybus L., Agrobacterium rhizogenes, genetic transformation, genes for tuberculosis antigenes ESAT6 and Ag85B or human interferon α2b

TSitologiya i Genetika
2011, vol. 45, no. 5, 11-16

Current Issue
Cytology and Genetics
2011, vol. 45, no. 5, 277-281,
doi: 10.3103/S0095452711050082

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