TSitologiya i Genetika 2011, vol. 45, no. 3, 20-25
Cytology and Genetics 2011, vol. 45, no. 3, 148-152, doi: https://www.doi.org/10.3103/S0095452711030030

Transgenic sugar beet tolerant to imidazolinone obtained by Agrobacterium-mediated transformation

Kishchenko E.M., Komarnitskii I.K., Kuchuk N.V.

Institute of Cell Biology & Genetic Engeneering Natl. Acad. Sci. of Ukraine, Kyiv

Sugar beet is highly sensitive to imidazolinone herbicides thus rotational restrictions exist. In order to develop imidazolinone tolerant sugar beets als gene from Arabidopsis thaliana encoding acetolactate synthase with S653N mutation was used for genetic transformation. Transgenic sugar beet plants were obtained by Agrobacterium-mediated transformation of aseptic seedlings using vacuum-infiltration. The efficiency of genetic transformation was 5.8 %. RT-PCR analysis of obtained plants revealed accumulation of specific als transcript. The resistance to imidazolinone was proved for developed transgenic sugar beet plants in vitro and in greenhouse conditions after spraying with imazethapyr (Pursuit®, BASF).

Keywords: Beta vulgaris L., herbicide resistance, genetic transformation, ALS (AHAS)

TSitologiya i Genetika
2011, vol. 45, no. 3, 20-25

Current Issue
Cytology and Genetics
2011, vol. 45, no. 3, 148-152,
doi: 10.3103/S0095452711030030

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