Main page
Contacts
Contents
Themes
Subscription
Information to authors
Editorial board
In Russian
UNIMARC
|  |
Nikolaiev Iu.S., Gilchuk P.V., Gorbatiuk O.B., Flyak A.I., Labyntsev A.J., Irodov D.M., Kolibo D.V., Kordium V.A.
Polyclonal antibodies against human cell-surface antigen CD34
TSitologiia i genetika 2011, v 45, Issue 3, 3-14
An efficient and inexpensive laboratory approach for the generation and the purification of polyclonal antibodies to human antigen CD34 was developed. It was shown that cloned refolded and purified from Escherichia coli recombinant extracellular fragment of CD34 antigen retained immunogenic determinants of cell-surface expressed CD34. Immunization of mice with unglycosylated truncated recombinant protein elicit polyclonal antibodies specific for the native human antigen CD34. The antibodies generated are applicable for phenotyping of CD34+ cells using immunocytochemistry and flow cytometry assays.
Nikolaiev Iu.S., Gilchuk P.V., Gorbatiuk O.B., Flyak A.I., Labyntsev A.J., Irodov D.M., Kolibo D.V., Kordium V.A. Polyclonal antibodies against human cell-surface antigen CD34 // Tsitol Genet. – 2011. – 45(3). – P. 3-14.
|
