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Creation of transgenic sugar beet lines expressing insect pest resistance genes cry1C and cry2A

Lytvyn D.I., Syvura V.V., Kurylo V.V., Olenieva V.D., Yemets A.I., Blume Ya.B.

 


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SUMMARY. Impact of insect pests makes a significant limitation of the sugar beet crop yield. Integration of cry-genes of Bacillus thuringiensis into plant genome is one of the promising strategies to ensure plant resistance. The aim of this work was to obtain sugar beet lines (based on the MM1/2 line) transformed with cry2A and cry1C genes. We have optimized transformation protocol and direct plant let regeneration protocol from leaf explants using 1 mg/l benzylaminopurine as well as 0,25 mg/l benzylamino-purine and 0,1 mg/l indole-butyric acid. Consequently, transgenic sugar beet lines transformed with vector cons-tructs pRD400-cry1C and pRD400-cry2A have been ob-tained. PCR analysis revealed integration of cry2A and cry1C into genome of transgenic lines and expression of these genes in leaf tissues was shown by reverse transcrip-tion PCR.

Tsitologiya i Genetika 2014, vol. 48, no. 2, pp. 3-11

E-mail: ldmi ukr.net

Lytvyn D.I., Syvura V.V., Kurylo V.V., Olenieva V.D., Yemets A.I., Blume Ya.B. Creation of transgenic sugar beet lines expressing insect pest resistance genes cry1C and cry2A, Tsitol Genet., 2014, vol. 48, no. 2, pp. 3-11.

In "Cytology and Genetics":
D. I. Lytvyn Email author , V. V. Syvura, V. V. Kurylo, V. D. Olenieva, A. I. Yemets, Ya. B. Blume Creation of transgenic sugar beet lines expressing insect pest resistance genes cry1C and cry2A, Cytol Genet., 2014, vol. 48, no. 2, pp. 69–75
DOI: 10.3103/S0095452714020078


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