TSitologiya i Genetika 2016, vol. 50, no. 3, 12-15
Cytology and Genetics 2016, vol. 50, no. 3, 158–161, doi: https://www.doi.org/10.3103/S0095452716030105

Mutagenesis testing using the LacZ reporter activity of the reparation gene mus209 in Drosophila melanogaster

Yasinskiy Y., Omelyanchuk L.V., Zhuk O.V., Kozeretska I.A.

  • Institute of Biology National Taras Shevchenko University, Kyiv
  • Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Science, Novosibirsk

We studied a set of Drosophila melanogaster strains that could be potentially suitable for testing a variety of mutagenic factors. Their genomes contained insertions of the enhancer trap P{lacW} in which the activity of the LacZ reporter is under the control of the reparation genes’ regulatory region. We demonstrated that the beta-galactosidase reporter, which is encoded by insertion of P{lacW} element in the gene mus209, is induced by irradiation in the cells of the salivary glands and wing imaginal discs. Despite the fact that the reporting coloration is not associated with the dose of radiation treatment, we found that the induction threshold of the reporter is different for these tissues. Thus, coloration in salivary glands is detectable after the dose of 200 rad and above, whereas the imaginal discs get colored with 500 rad and above. Thereby, multiple thresholds for induction of the reporter in the various tissues allow approximating the received dose.

Keywords: mutagenic factors, D. melanogaster, LacZ reporter, radiation

TSitologiya i Genetika
2016, vol. 50, no. 3, 12-15

Current Issue
Cytology and Genetics
2016, vol. 50, no. 3, 158–161,
doi: 10.3103/S0095452716030105

Full text and supplemented materials

References