TSitologiya i Genetika 2016, vol. 50, no. 4, 79-89
Cytology and Genetics 2016, vol. 50, no. 4, 257–266, doi: https://www.doi.org/10.3103/S0095452716040022

Chromosomal DNA balance in human stem cell line 4BL

Akopyan H.R., Kushniruk V.O., Mykytenko D.O., Huleuk N.L., Kremenskaya Y., Lukash L.L.

SUMMARY. In the previous cytogenetic study of new human stem cell line 4BL at the 205th passage we observed the ploidy of chromosomal set and regular aberrations. To investigate the nature of monosomy of certain chromosomes the array CGH and FISH analyses have been used. The aberrations of chromosomes have been identified in all the cases of monosomies previously revealed by G-banding. The largest changes of the DNA balance have been detected in the chromosomes 2, 4, 10, 13 and 17. The probable cause of the monosomies of chromosomes 4, 10, 13 and 17 is massive loss of the genetic material. The monosomy of the second chromosome pair is caused by significant transformation one of the homologs in a type of numerous duplications and formation of der(2)t(2;?)(q21;?). Due to application of array CGH the regions of the structural aberrations of the chromosomes 2, 4, 10, 13 and 17 have been concretized, what permitted to perform their clarifying identification by multicolored FISH method. The results obtained by us confirm the hypothesis about coordinated appearance of the deletions and duplications and their stabilization impact on the transformed chromosomes.

Keywords: stem cell line in vitro, chromosome aberrations, DNA balance, FISH, array CGH

TSitologiya i Genetika
2016, vol. 50, no. 4, 79-89

Current Issue
Cytology and Genetics
2016, vol. 50, no. 4, 257–266,
doi: 10.3103/S0095452716040022

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