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Accumulation of indole-3-acetic and abscisic acid in transformed roots of Artemisia vulgaris

Kosakivska I.V., Voytenko L.V., Drobot K.O., Matvieieva N.A.

 




SUMMARY. The character of accumulation and balance of endogenous indole-3-acetic and abscisic acids in Artemisia vulgaris roots transformed by Agrobacterium rhizogenes A4 was analyzed by high performance liquid chro-matography-mass spectrometry method. It was shown that the content of free IAA significantly exceeded the level of the conjugated form. The highest amount of active form of IAA (554,4 27,7 ng/g of fresh weight) was recorded in line number 2. In roots of control samples dominated the free form of ABA. Its level was significantly higher than in the transformed lines. Bearded roots accumulated a conjugated form of ABA, the highest content of which in line number 4 reached 459,6 23,0 ng/g of fresh weight. The architecture of the transformed roots was marked by significant branching, the formation of lateral roots; active accumulation of biomass is noted. The results showed changes in the balance of endogenous phytohormones in the bearded roots of A. vulgaris in the direction of domination of the free form of IAA in the context of a significant decrease of ABA.

SUMMARY. The character of accumulation and balance of endogenous indole-3-acetic and abscisic acids in Artemisia vulgaris roots transformed by Agrobacterium rhizogenes A4 was analyzed by high performance liquid chro-matography-mass spectrometry method. It was shown that the content of free IAA significantly exceeded the level of the conjugated form. The highest amount of active form of IAA (554,4 27,7 ng/g of fresh weight) was recorded in line number 2. In roots of control samples dominated the free form of ABA. Its level was significantly higher than in the transformed lines. Bearded roots accumulated a conjugated form of ABA, the highest content of which in line number 4 reached 459,6 23,0 ng/g of fresh weight. The architecture of the transformed roots was marked by significant branching, the formation of lateral roots; active accumulation of biomass is noted. The results showed changes in the balance of endogenous phytohormones in the bearded roots of A. vulgaris in the direction of domination of the free form of IAA in the context of a significant decrease of ABA.

Tsitologiya i Genetika 2018, vol. 52, no. 6, pp. 3-9

E-mail: irynakosakivska gmail.com, lesyavoytenko gmail.com, katyadrobot gmail.com, joyna56 gmail.com

Kosakivska I.V., Voytenko L.V., Drobot K.O., Matvieieva N.A. Accumulation of indole-3-acetic and abscisic acid in transformed roots of Artemisia vulgaris, Tsitol Genet., 2018, vol. 52, no. 6, pp. 3-9.

In "Cytology and Genetics":
I. V. Kosakivska, L. V. Voytenko, K. O. Drobot, N. A. Matvieieva Accumulation of Indolyl-3-Acetic and Abscisic Acids by Hairy Roots of Artemisia vulgaris, Cytol Genet., 2018, vol. 52, no. 6, pp. 395399
DOI: 10.3103/S0095452718060051


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