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Investigation of allilic diversity of HMW glutenins genes of wheat varieties and lines used in the breeding process in the republic of Belarus by PCR markers
SUMMARY. The allelic composition of genes encoding glutenins was studied in the collection of 236 winter wheat samples and 98 spring wheat samples of Belarusian and foreign breeding. 13 alleles among winter wheat samples and 11 alleles among spring wheat samples were revealed. This is generally less than in European, Asian and African countries. 36 different genotypes were identified among studied samples. Varieties and lines with genotypes Glu-A1c, Glu-B1c, Glu-D1d (16,2 % of the total number of studied samples), Glu-A1a, Glu-B1c, Glu-D1d (13,7%), Glu-A1b, Glu-B1c, Glu-D1d (12,7 %) are the most frequent among winter wheat samples. Varieties and lines with genotypes Glu-A1a, Glu-B1c, Glu-D1d (22,5 %), Glu-A1a, Glu-B1c, Glu-D1a (16,4 %), Glu-A1c, Glu-B1f, Glu-D1d (13,3 %) prevail among spring wheat samples. The allele of the gene encoding the primary structure of the Bx-subunit with a maximum identity degree 99 % relatively to the Bx-subunits represented in the GenBank database was revealed in the spring wheat line KP-406/11 genome. It contains an open reading frame with a length of 2367 bp and has the highest identity degree with the nucleotide sequence of the Bx14-subunit. The 789 amino acid sequence encoded by this allele differs from Bx14 by replacement of amino acid residues in three positions – 662, 780 and 788. It was designated as Bx14.1 (accession number in Genbank MH108092).
E-mail: E.Fomina igc.by, S.Malyshev igc.by, xaccka gmail.com, cytoplasmic mail.ru, O.Urbanovich igc.by
1. Halford, N.G., Field, J.M., Blair, H., Urwin, P., Moore, K., Robert, L., Thompson, R., Flavell, R.B., Tatham, A.S., and Shewry, P.R., Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality, Theor. Appl. Genet., 1992, vol. 83, pp. 373–378. https://doi.org/10.1007/BF00224285
2. Shao, H., Liu, T.-H., Ran, C.-F., Li, L.Q., Yu, J., Gao, X., and Li, X.-J., Isolation and molecular characterization of two novel HMW-GS genes from Chinese wheat (Triticum aestivum L.) landrace Banjiemang, Genes Genom., 2015, vol. 37, pp. 45–53. https://doi.org/10.1007/s13258-014-0228-3
3. Payne, P.I., Corfield, K.G., and Blackman, J.A., Identification of a high molecular weight subunit of glutenin whose presence correlated with breadmaking quality in wheats of related pedigree, Theor. Appl. Genet., 1979, vol. 55, pp. 153–159. https://doi.org/10.1007/BF00295442
4. Gale, K.R., Diagnostic DNA markers for quality traits in wheat, J. Cereal Sci., 2004, vol. 41, pp. 181–192. https://doi.org/10.1016/j.jcs.2004.09.002
5. Bekes, F. and Wrigley, C.W., Gluten alleles and predicted dough quality for wheat varieties worldwide: a great resource—free on the AACC international website, Cereal Foods World, 2013, vol. 58, no. 6, pp. 325–328. https://doi.org/10.1094/CFW-58-6-0325
6. Ma, W., Zhang, W., and Gale, K.R., Multiplex-PCR typing of high molecular weight glutenin alleles in wheat, Euphytica, 2003, vol. 134, pp. 51–60. https://doi.org/10.1023/A:1026191918704
7. Laflandra, D., Tucci, G.F., Pavoni, A., Turchetta, T., and Margiotta, B., PCR analysis of x- and y-type genes present at the complex Glu-A1 locus in durum and bread wheat, Theor. Appl. Genet., 1997, vol. 94, pp. 235–240. https://doi.org/10.1007/s001220050405
8. Yan, Z., Dai, S., Liu, D., Wei, Y., Wang, J., and Zheng, Y., Isolation and characterization of a novel Glu-Bx HMW-GS allele from Tibet bread wheat landrace, Int. J. Agric. Res., 2009, vol. 4, no. 1, pp. 38–45. https://doi.org/10.3923/ijar.2009.38.45
9. Payne, P.I., Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality, Ann. Rev. Plant Physiol., 1987, vol. 38, pp. 141–153. https://doi.org/10.1146/annurev.pp.38.060187.001041
10. Ahmad, M., Molecular marker-assisted selection of HMW glutenin alleles related to wheat bread quality by PCR-generated DNA markers, Theor. Appl. Genet., 2000, vol. 101, pp. 892–896. https://doi.org/10.1007/s001220051558
11. Tishchenko, V.N., Chekalin, N.M., Panchenko, I.A., and Didenko, S.Yu., Polymorphism of glutenins in the varieties of winter wheat of the Poltava selection, Visn. Poltava State Agrar. Acad., 2006, vol. 3, pp. 5–9.
12. Morgun, V.V., Tarasyuk, O.I., Pochinok, V.M., and Rybalka, A.I., Original genetic variability in alleles of Glu loci for wheat selection for grain quality, Proc. BSU, 2014, vol. 9, no. 1, pp. 141–147.
13. Kozub, N.A., Sozinov, I.A., Sobko, T.A., Kolyuchii, V.T., Kuptsov, S.V., and Sozinov, A.A., Variation at storage protein loci in winter common wheat cultivars of the central forest–steppe of Ukraine, Cytol. Genet., 2009, vol. 43, no. 1, pp. 55–62. https://doi.org/10.3103/S0095452709010101
14. Moczulski, M. and Salmanowicz, B.P., Multiplex PCR identification of wheat HMW glutenin subunit genes by allele-specific markers, J. Appl. Genet., 2003, vol. 44, no. 4, pp. 459–471.
15. Fan, X., Song, Z.J., Kang, H.Y., Yang, R.W., and Zhou, Y.H., Identification and characterization of HMW glutenin subunits and their coding sequences in dwarfing Polish wheat, Int. J. Agric. Res., 2009, vol. 4, no. 8, pp. 237–249. https://doi.org/10.3923/ijar.2009.237.249
16. Tsenov, N., Atanasova, D., Todorov, I., Ivanova, I., and Stoeva, I., Allelic diversity in Bulgarian winter wheat varieties based on polymorphism of glutenin subunit composition, Cer. Res. Commun., 2009, vol. 37, no. 4, pp. 551–558. https://doi.org/10.1556/CRC.37.2009.4.8
17. Atanasova, D., Tsenov, N., Todorov, I., and Ivanova, I., Glutenin composition of winter wheat varieties bred in Dobrudzna Agricultural Institute, Bulgar. J. Agric. Sci., 2009, vol. 15, no. 1, pp. 9–19.
18. Vaiciulyte-Funk, L., Juodeikiene, G., and Bartkiene, E., The relationship between wheat baking properties, specific high molecular weight glutenin components and characteristics of varieties, Zemdirbyste-Agric., 2015, vol. 102, no. 2, pp. 229–238. https://doi.org/10.13080/za.2015.102.030
19. Bradová, J. and Štočková, L., Evaluation of winter wheat collection in terms of HMW- and LMW glutenin subunits, Czech J. Genet. Plant Breed., 2010, vol. 46, pp. 96–99. https://doi.org/10.17221/2448-CJGPB
20. Chňapek, M., Tomka, M., Peroutková, R., and Gálová, Z., Polymorphism of HMW-GS in collection of wheat genotypes, Int. J. Biol., Biomol. Agric. Food Biotechnol. Eng., 2014, vol. 8, no. 7, pp. 652–657.
21. Baracskai, I., Balázs, G., Li, L., Ma, W., Oszvald, M., Newberry, M., Tomoskozi, S., Láng, L., Bedö, Z., and Békés, F., A retrospective analysis of HMW and LMW glutenin alleles of cultivars bred in Martonvasar, Cereal Res. Commun., 2011, vol. 39, no. 2, pp. 225–236. https://doi.org/10.1556/CRC.39.2011.2.6
22. Branlard, G., Dardevet, M., Amiour, N., and Igrejas, G., Allelic diversity of HMW and LMW glutenin subunits and omega-gliadins in French bread wheat (Triticum aestivum L.), Gen. Res. Crop Evol., 2003, vol. 50, pp. 669–679. https://doi.org/10.1023/A:1025077005401
23. Zarghani, E. and Imamjomeh, A., Assesment of genetic diversity in high-molecular-weight glutenin subunits and relationship to breed-making quality in common wheat, Trakia J. Sci., 2011, vol. 9, no. 1, pp. 37–42.
24. Tahir, N.A., Evaluation of hexaploid wheat varieties for making bread by high molecular weight (HMW) and low molecular weight (LMW) analysis, Jordan J. Biol. Sci., 2009, vol. 2, pp. 55–62.
25. Tabasum, A., Iqbal, N., Hameed, A., and Arshad, R., Evaluation of Pakistani wheat germplasm for bread quality based on allelic variation in HMW glutenin subunits, Pak. J. Bot., 2011, vol. 43, no. 3, pp. 1735–1740.
26. Yasmeen, F., Khurshid, H., and Ghafoor, A., Genetic divergence for high-molecular weight glutenin subunits (HMW-GS) in indigenous landraces and commercial cultivars of bread wheat of Pakistan, Genet. Mol. Res, 2015, vol. 14, no. 2, pp. 4829–4839. https://doi.org/10.4238/2015.May.11.15
27. Kaur, A., Singh, N., Ahlawat, A.K., Kaur, S., Singh, A.M., Chauhan, H., and Singh, G.P., Diversity in grain, flour, dough and gluten properties amongst Indian wheat cultivars varying in high molecular weight subunits (HMW-GS), Food Res. Int., 2013, vol. 53, pp. 63–72. https://doi.org/10.1016/j.foodres.2013.03.009
28. He, Z.H., Liu, L., Xia, X.C., Liu, J.J., and Pena, R.J., Composition of HMW and LMW glutenin subunits and their effects on dough properties, pan bread, and noodle quality of Chinese bread wheats, Cereal Chem., 2005, vol. 82, no. 4, pp. 345–350. https://doi.org/10.1094/CC-82-0345
29. Liu, Y., Xiong, Z.Y., He, Y.G., Shewry, P.R., and He, G.Y., Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province, Genet. Res. Crop Evol., 2007, vol. 54, pp. 865–874. https://doi.org/10.1007/s10722-006-9154-9
30. Dessalegn, T., Van Deventer, C.S., Labuschagne, M.T., and Martens, H., Allelic variation of HMW glutenin subunits of Ethiopian bread wheat cultivars and their quality, Afr. Crop Sci. J., 2011, vol. 19, no. 2, pp. 55–63. https://doi.org/10.4314/acsj.v19i2.69855
31. Bellil, I., Chekara Bouziani, M., and Khelifi, D., Genetic diversity of high and low molecular weight glutenin subunits in Saharan bread and durum wheats from Algerian oases, Czech. J. Genet. Plant Breed., 2012, vol. 48, no. 1, pp. 23–32. https://doi.org/10.17221/105/2011-CJGPB
32. Plaschke, J., Ganal, M.W., and Roder, M.S., Detection of genetic diversity in closely related bread wheat using microsatellite markers, Theor. Appl. Genet., 1995, vol. 91, pp. 1001–1007. https://doi.org/10.1007/BF00223912
33. Liu, S., Chao, S., and Anderson, J.A., New DNA markers for high molecular weight glutenin subunits in wheat, Theor. Appl. Genet., 2008, vol. 118, no. 1, pp. 177–183. https://doi.org/10.1007/s00122-008-0886-0
34. Schwarz, G., Felsenstein, F.G., and Wenzel, G., Development and validation of a PCR-based marker assay for negative selection of the HMW glutenin allele Glu-B1-1d (Bx-6) in wheat, Theor. Appl. Genet., 2004, vol. 109, no. 5, pp. 1064–1069. https://doi.org/10.1007/s00122-004-1718-5
35. Ragupathy, R., Naeem, H.A., Reimer, E., Lukow, O.M., Sapirstein, H.D., and Cloutier, S., Evolutionary origin of the segmental duplication encompassing the wheat GLU-B1 locus encoding the overexpressed Bx7 (Bx7OE) high molecular weight glutenin subunit, Theor. Appl. Genet., 2008, vol. 116, pp. 283–296. https://doi.org/10.1007/s00122-007-0666-2
36. Lei, Z.S., Gale, K.R., He, Z.H., Gianibelli, C., Larroque, O., Xia, X.S., Butow, B.J., and Ma, W., Y-type gene specific markers for enhanced discrimination of high-molecular weight glutenin alleles at the Glu-B1 locus in hexaploid wheat, J. Cer. Sci., 2006, vol. 43, pp. 94–101. https://doi.org/10.1016/j.jcs.2005.08.003
37. Payne, P.I., Nigtingale, M.A., Krattiger, A.F., and Holt, L.M., The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties, J. Sci. Food Agric., 1987, vol. 40, pp. 51–65. https://doi.org/10.1002/jsfa.2740400108
38. Rogers, W.J., Payne, P.I., and Harinder, K., The HMW glutenin subunit and gliadin compositions of Germany-grown wheat varieties and their relationship with bread-making quality, Plant Breed., 1989, vol. 103, pp. 89–100. https://doi.org/10.1111/j.1439-0523.1989.tb00356.x
39. Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Mentjies, P., and Drummond, A., Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data, Bioinformatics, 2012, vol. 28, no. 12, pp. 1647–1649. https://doi.org/10.1093/bioinformatics/bts199
40. Bekes, F., Cavanagh, C.R., Martinov, S., Bushuk, W., and Wrigley, C.W., The Gluten Composition of Wheat Varieties and Genotypes Part II. Composition Table for the HMW Subunits of Glutenin, 3rd ed.
41. McIntosh, R.A., Hart, G.E., Devos, K.M., Gale, M.D., and Rogers, W.J., Catalogue of Gene Symbols for Wheat, 1998.
42. Dobrotvorskaya, T.V. and Martynov, S.P., Analysis of diversity of Russian and Ukrainian bread wheat (Triticum aestivum L.) cultivars for high-molecular-weight glutenin subunits, Russ. J. Genet., 2011, vol. 47, no. 7, pp. 799–812. https://doi.org/10.1134/S1022795411070052
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