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Effect of platelet rich plasma on morphogenesis and chondrogenic marker gene expression by chondrocyte-like rat Nucleus pulposus cells in vitro

Pedachenko E.G., Vasilyeva I.G., Khizniak M.V., Chopyck N.G., Oleksenko N.P., Shuba I.N., Tsjubko O.I., Galanta O.S., Dmytrenko A.B., Makarova T.A., Snitsar N.D.

 




SUMMARY. The purpose was to study the effect of 3, 10 and 20 % rat platelet rich plasma (PRP) on the morphobiologic characteristics of chondrocyte-like rat nucleus pulposus (NP) cells, and also expression of chondrogenic genes: ACAN, COL II, GPC3, MGP, PTN, ANXA3; VIM in vitro. The viability of rat NP cells in culture is dose-dependently enhanced by 3, 10, 20 % PRP. Platelets in cultures show tropicity to blast-like cells, form an adhesion zone. When 20 % PRP are used at the 14th day of cultivation, the blast-like cells are differentiated, the cells are recorded at the stage of division. The decreased expression of chondrogenic genes is dose-dependently prevented by PRP. More effective was 20 % PRP. In these cultures, an increase in the expression of most studied chondrogenic genes was observed: 2,9 times higher for COL II (P = 0,002); 2,1 times higher for VIM (P = 0.0003); 2,3 times higher for ANXA (P = 0,0003); 1,4 times higher for GPC (P = 0.0011); 1,6 times higher for PTN (P = 0,0017), 1,2 times higher for ACAN (P = 0,0606).

Tsitologiya i Genetika 2019, vol. 53, no. 2, pp. 34-42

E-mail: pedach i.ua, vigvasileva gmail.com, Khyzhnyak63 gmail.com, natalia.chopyck gmail.com, n.oleksenko gmail.com, irina_shuba ukr.net, tsjubkoolga gmail.com, lenagalanta gmail.com, 1866600 online.ua, tatyana.makarova77799 gmail.com, angelikdmiz.bor gmail.com

Pedachenko E.G., Vasilyeva I.G., Khizniak M.V., Chopyck N.G., Oleksenko N.P., Shuba I.N., Tsjubko O.I., Galanta O.S., Dmytrenko A.B., Makarova T.A., Snitsar N.D. Effect of platelet rich plasma on morphogenesis and chondrogenic marker gene expression by chondrocyte-like rat Nucleus pulposus cells in vitro, Tsitol Genet., 2019, vol. 53, no. 2, pp. 34-42.

In "Cytology and Genetics":
E. G. Pedachenko, I. G. Vasilyeva, M. V. Khizniak, N. G. Chopyck, N. P. Oleksenko, I. N. Shuba, O. I. Tsjubko, O. S. Galanta, N. D. Snitsar, T. A. Makarova, A. B. Dmytrenko The Effect of Platelet-Rich Plasma on Morphogenesis and Expression of Chondrogenic Marker Genes by Chondrocyte-Like Cells of the Rat Nucleus Pulposus in Vitro, Cytol Genet., 2019, vol. 53, no. 2, pp. 124–131
DOI: 10.3103/S0095452719020087


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