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Genetic modification of Saccharum officinarum for herbicide tolerance

Muhammad Fahad Khan, Qurban Ali, Muhammad Tariq, Shafique Ahmed, Zahida Qamar, Idrees Ahmad Nasir

 




Sugarcane is one of the most important worldwide cultivated agro-industrial crops that belong to the family Poaeceae and genus Saccharum. Prescribed study was conducted in Seed Biotechnology Lab, Center of Excellence in Molecular Biology, University of the Punjab, Lahore-Pakistan. The purpose of this study was to alter the genome of sugarcane line CPF246 with herbicide resistance gene GTGene driven by the sugar cane ubiquitin promoter and confirmation through PCR and Dot blot as well as protein expression analysis through ELISA. Results have shown the successful transformation and expression of transgenes against glyphosate resistance. This could prove a mile stone towards putting research efforts in the improvement of saccharum officinarum for certain traits. Outcomes of this research will be beneficial in minimizing the management cost which people do bear for weed management practices. Success of this study opens new horizons to further improve other characteristics of sugarcane using genetic modification as was done in current study.

Key words: Saccharum officinarum, genetic modification, herbicide, GTGene, Glyphosate

Tsitologiya i Genetika 2019, vol. 53, no. 3, pp. 72-74

  1. Centre of Excellence in Molecular Biology, University of the Punjab Lahore, Pakistan
  2. Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore Pakistan

E-mail: saim1692 gmail.com

Muhammad Fahad Khan, Qurban Ali, Muhammad Tariq, Shafique Ahmed, Zahida Qamar, Idrees Ahmad Nasir Genetic modification of Saccharum officinarum for herbicide tolerance, Tsitol Genet., 2019, vol. 53, no. 3, pp. 72-74.

In "Cytology and Genetics":
Muhammad Fahad Khan, Qurban Ali, Muhammad Tariq, Shafique Ahmed, Zahida Qamar, Idrees Ahmad Nasir Genetic Modification of Saccharum officinarum for Herbicide Tolerance, Cytol Genet., 2019, vol. 53, no. 3, pp. 239–249
DOI: 10.3103/S0095452719030101


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