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Calcium and components of lipid signaling in realization of hydrogen sulfide influence on state of stomata in Arabidopsis thaliana

Yastreb Т.О., Kolupaev Yu.Е., Havva E.N., Shkliarevskyi M.A., Dmitriev А.P.

 




SUMMARY. A time and concentration dependence of the hydrogen sulfide donor sodium hydrosulfide (NaHS) influence on the state of stomata of Arabidopsis thaliana (Col-0) leaves, as well as the role of calcium and phospholipases in the realization of its effects, was studied. Treatment of leaves with NaHS in concentration range of 5–250 μM caused a decrease in the size of stomatal aperture. The maximal effect of stomatal closure was observed 90 minutes after the beginning of the H2S donor treatment and after 180 minutes of exposure the stomatal aperture in NaHS variants was, on the contrary, much wider than in the control. The effect of leaves’ treatment with NaHS solutions on the stomata state was completely eliminated by hydroxylamine, the hydrogen sulfide scavenger, which indicates the specificity of NaHS effects as a H2S donor. The decrease in stomatal aperture and relative number of open stomata caused by the donor of hydrogen sulfide was almost completely leveled off by the pre-treatment of leaves with the calcium channel blocker lanthanum chloride, the extracellular calcium chelator EGTA, the phospholipase C inhibitor neomycin, and the antagonist of the cyclic adenosine-5'-diphosphate ribose formation nicotinamide. Also, the stomatal effect of the H2S donor was partially eliminated by the calmodulin antagonist chlorpromazine. The leveling of the hydrogen sulfide donor action on the state of stomatal apparatus of Arabidopsis leaves was also noted at the pre-treatment of leaves with butanol-1, an inhibitor of phospholipase D-dependent formation of phosphatidic acid. A conclusion was made about the value of calcium intake into the cytosol from various compartments, as well as lipid signaling mediators formed with the participation of phospholipases C and D, in the action of hydrogen sulfide on the state of stomata.

Key words: Arabidopsis thaliana, stomata, hydrogen sulfide, calcium, lipid signaling

Tsitologiya i Genetika 2019, vol. 53, no. 2, pp. 3-11

E-mail: plant_biology ukr.net, dmitriev.ap gmail.com

Yastreb Т.О., Kolupaev Yu.Е., Havva E.N., Shkliarevskyi M.A., Dmitriev А.P. Calcium and components of lipid signaling in realization of hydrogen sulfide influence on state of stomata in Arabidopsis thaliana, Tsitol Genet., 2019, vol. 53, no. 2, pp. 3-11.

In "Cytology and Genetics":
T. O. Yastreb, Yu. E. Kolupaev, E. N. Havva, M. A. Shkliarevskyi, A. P. Dmitriev Calcium and Components of Lipid Signaling in Implementation of Hydrogen Sulfide Influence on the State of Stomata in Arabidopsis thaliana, Cytol Genet., 2019, vol. 53, no. 2, pp. 99–105
DOI: 10.3103/S0095452719020099


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