The use of intergeneric conjugation Escherichia coli – Streptomyces for transfer of recombinant DNA into S.nogalater IMET43360 strain

Klimishin D.O., Gromyko O.M., Fedorenko V.O.

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Successful transfer of the studied plasmids into S. nogalater IMET43360 cells using bacterial conjugation in the system E. coli – Streptomyces is an appropriate method for constructing this strain. Using DNA–DNA hybridization the character of integration of pVWB and pRT801 plasmids has been studied. The influence of these plasmids on nogalamycin biosynthesis has been investigated as well. The obtained results enable detailed study of nogalamycin gene functioning in S.nogalater IMET43360. The use of conjugation for substitution, destruction of genes, and heterological expression allows to get new «hybrid» antibiotics produced by this strain.

Tsitologiya i Genetika 2007, vol. 41, no. 5, pp. 3-8

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Klimishin D.O., Gromyko O.M., Fedorenko V.O. The use of intergeneric conjugation Escherichia coli – Streptomyces for transfer of recombinant DNA into S.nogalater IMET43360 strain, Tsitol Genet., 2007, vol. 41, no. 5, pp. 3-8.