Heterologeus expression of lndYR and wblA_gh genes in producers of anthracyclin antibiotics Streptomyces nogalater Lv65, S. echinatus DSM40730 and S. peucetius subsp. caesius ATCC27952

Klymyshin D.O., Nimets O.Ya., Stefanyshyn O.M., Fedorenko V.O.

SUMMARY. Strains of actinomycetes Streptomyces nogalater Lv65, S. echinatus DSM40730, and S. peucetius subsp. caesius ATCC27952 are producers of anthracycline antibiotics nogalamycine, aranciamycine and doxorubicin, respectively. Here we have focused on the impact of lndYR and wblA_gh regulatory genes expression on secondary metabolism and morphogenesis of these bacteria. Expression of regulators decreased the synthesis of anthracyclines that may indicate the presence of their homologues in the genomes. In S. peucetius subsp. caesius ATCC27952 lndYR also affect sporulation. Directed inactivation of these genes can be a successful tool for generation of strains with elevated synthesis of clinically important compounds, as well as elucidation of regulatory stages in biosynthesis of anthracycline antibiotics.

Tsitologiya i Genetika 2013, vol. 47, no. 4, pp. 3-8

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Klymyshin D.O., Nimets O.Ya., Stefanyshyn O.M., Fedorenko V.O. Heterologeus expression of lndYR and wblA_gh genes in producers of anthracyclin antibiotics Streptomyces nogalater Lv65, S. echinatus DSM40730 and S. peucetius subsp. caesius ATCC27952, Tsitol Genet., 2013, vol. 47, no. 4, pp. 3-8.