TSitologiya i Genetika 2022, vol. 56, no. 6, 3-18
Cytology and Genetics 2022, vol. 56, no. 6, 481–493, doi: https://www.doi.org/10.3103/S009545272206007X

Characterization of β-tubulin genes in Prunus persica and Prunus dulcis for fingerprinting of their interspecific hybrids

Lykholat Y.V., Rabokon A.M., Blume R.Ya., Khromykh N.O., Didur O.O., Sakharova V.H., Kabar A.M., Pirko Ya.V., Blume Ya.B.

  1. Oles Honchar Dnipro National University, Gagarin Ave., 72, Dnipro, 49010, Ukraine
  2. Institute of Food Biotechnology and Genomics of National Academy of Sciences of Ukraine, Osypovskoho st., 2a, 04123, Kyiv, Ukraine

SUMMARY. Peach is one of the most important fruit crops, cultivation of which held the third largest areas among all fruit crops, grown in temperate climate zone. Cultivation of this crop under less favourable clime conditions would require the creation of new resistant genotypes via the intra- or interspecific hybridization, including crossing with almond. Efficient breeding of hybrids and their long-term selection will require a rapid and accurate method of genetic barcoding that would be able to distinguish closely-related genotypes or to detect interspecific hybrids. One such approaches is TBP-analysis, which is based on the evaluation of intron length polymorphism of β-tubulin genes. However, the correct interpretation of the results of such an analysis should be based on data on the diversity of β-tubulin genes panel in the genomes of the analyzed species. Thus, here we report on the successful whole-genome identification and on comprehensive analysis of the phylogeny and synteny of the β-tubulin genes of P. persica and P. dulcis, as well as we demonstrate the the possibility to use such data of the genomic search for interpretation of data of TBP genotyping of intra- and interspecific hybrids of peach and almond species. In general, 11 β-tubulin genes were indentified within the P. persica genome, and 10 genes, in the P. dulcis genome, accounting pseudogenes. Additionally, phylogenetic and synteny analyses of the identified genes made it possible to identify the orthologues in the genomes of A. thaliana and A. lyrata, as well as to designate identified β-tubulins to specific isotypes. Genotyping via the TBP-method allowed obtaining distinct molecular profiles for 11 investigated accessions, among which 8 were intra- or interspecific hybrids. Based on obtained results of genotyping, a cluster analysis was carried out, the results of which correlated well with the breeding history of the analysed genotypes, what additionally confirmed the effectiveness and accuracy of the genotyping approach used.

Keywords: peach, almond, interspecific hybrids, ILP, tubulin genes, genotyping, pathogen resistance

TSitologiya i Genetika
2022, vol. 56, no. 6, 3-18

Current Issue
Cytology and Genetics
2022, vol. 56, no. 6, 481–493,
doi: 10.3103/S009545272206007X

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