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Karyotypic study of five lutjanid species using conventional and Ag­NORs banding techniques

Phimphan S., Supiwong W., Tanomtong A., Pinthong K., Saowakoon S., Kaewsri S.

The first cytogenetic comparisons of five snapper spe­cies from Thailand were presented here. Renal cell samples were taken from blacktail snapper (Lutjanus fulvus), five lined snapper (L. quinquelineatus), dory snapper (L. fulviflamma), brownstripe red snapper (L. vitta), and mangrove red snapper (L. argentimaculatus). The mitotic chromosome preparation was prepared di­
rectly from kidney cells. Conventional staining and Ag­NOR banding techniques were applied to stain the chromosomes. The results exhibited that all five snapper species have the diploid chromosome numbers of 2n = 48 and the fundamental numbers (NF) of 48. The presences of large, medium, and small telocentric chromosomes were 22­24­2, 24­20­4, 36­10­2, 28­16­4 and 36­10­2, respectively. The Ag­NORs banding technique provides the pair of nucleolar organizer regions (NORs) at subcentromeric region of the long arm of the respective telocentric chromosome pairs 9, 1, 3, 4 and 9. Their karyotype formulas is as follows: L. fulvus (2n = 48): L
t₂₂+M
t₂₄+S
t₂, L. quinquelineatus (2n = 48): L
t₂₄+M
t₂₀+S
t₄, L. fulviflamma (2n = 48): L
t₃₆+M
t₁₀+S
t₂, L. vitta (2n = 48): L
t₂₈+M
t₁₆+S
t₄, and L. argentimaculatus (2n = 48): L
t₃₆+M
t₁₀+S
t₂.

Key words: Lutjanus, Chromosome, Karyotype, Nucleolar organizer regions

Tsitologiya i Genetika 2017, vol. 51, no. 4, pp. 81-83

• Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002, Thailand
• Department of Fundamental Science, Faculty of Science and Technology, Surindra Rajabhat University, Muang, Surin 32000, Thailand
• Faculty of Agriculture and Technology, Rajamangala University of Technology Issan Surin Campus, Muang, Surin 32000, Thailand
• Biology Program, Department of Science, Faculty of Science, Buriram Rajabhat University, Muang, Buriram 31000, Thailand

E-mail: tanomtong hotmail.com

Phimphan S., Supiwong W., Tanomtong A., Pinthong K., Saowakoon S., Kaewsri S. Karyotypic study of five lutjanid species using conventional and Ag­NORs banding techniques, Tsitol Genet., 2017, vol. 51, no. 4, pp. 81-83.