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SUMMARY. It was shown that for efficient production of regenerants in bread wheat, 18-day old calli, obtained from apical meristems of 3-day-old aseptic seedlings should be used. These calli show proliferative centers, with developed vascular system elements, that support the development of meristematic centers which later produce regenerants. It was shown that regeneration from the calli occurs by direct organogenesis but not by somatic embryogenesis. Under the influence of the antibiotic ceftriaxone, added to the regeneration medium in concentration of 400 mg/l, two different bread wheat genotypes showed, firstly, changes in apical origin callus morphology; secondly, an intensification of the regeneration processes; thirdly, roots development in the regenerants, thereby it allows to avoid the additional step of plants rooting.
