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Estimation of the genetic structure of Helix pomatia L. populations (Mollusca, Pulmonata) by capillary electrophoresis of ISSR DNA fragments

Snegin E.A., Artemchuk O.Yu., Sychev A.A.

SUMMARY. Using the capillary electrophoresis of ISSR DNA fragments, the genetic structure of the adventitious populations of Helix pomatia L. (Mollusca, Pulmonata) inhabiting the territory of Eastern Europe was studied. A significant decrease in genetic heterogeneity (H_e = 0.095 ± 0.002) and population differentiation level (Ф_st = 0.074) was revealed in comparison with similar data obtained using the ISSR method with electrophoretic detection in an agarose gel and allozymes in a polyacrylamide gel. Similar to other methods, an isolation effect of distance (R_M = –0.476) was found on the basis of regression analysis between genetic and geographical distances. The effective population number, calculated with the help of M. Slatkin’s model, turned out to be the largest of the previously calculated values (N_e= 41.8), which indicates the high viability of the studied groups of Roman snails in newly developed territories.

Key words: capillary electrophoresis, populations, genetic structure, terrestrial mollusk

Tsitologiya i Genetika 2018, vol. 52, no. 2, pp. 33-38

E-mail: snegin bsu.edu.ru

Snegin E.A., Artemchuk O.Yu., Sychev A.A. Estimation of the genetic structure of Helix pomatia L. populations (Mollusca, Pulmonata) by capillary electrophoresis of ISSR DNA fragments, Tsitol Genet., 2018, vol. 52, no. 2, pp. 33-38.

In "Cytology and Genetics":
E. A. Snegin, O. Yu. Artemchuk, A. A. Sychev Estimation of the Genetic Structure of Helix pomatia L. Populations (Mollusca, Pulmonata) by Capillary Electrophoresis of ISSR DNA Fragments, Cytol Genet., 2018, vol. 52, no. 2, pp. 112–116
DOI: 10.3103/S0095452718020093