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The role of post-translational acetylation in the association of ATG8 autophagia protein with microtubules from plant cells

Rayevsky A., Ozheredov D.S., Samofalova D., Ozheredov S.P., Karpov P.А., Blume Ya.B.

SUMMARY. At the moment, a number of factors are known that can influence the processes of the cell cycle. One of these factors are post-translational modifications (PTMs) of proteins that cause changes in their structure and, as a consequence, functionality and ability to interact with other proteins of the cell, including microtubule proteins. The aim of our work was a comparative analysis of the mechanism of the effect of PTMs on the structure and activity of proteins by the example of the effect of acetylation of Lys40 in α-tubulin TUBA4 from A. thaliana on its interaction with ATG8а. All the studied proteins were reconstructed by homology to the experimentally proven crystal structures. Further comparative analysis of protein-protein interactions was carried out using in silico methods. We have shown that acetylation of α-tubulin at Lys40 leads to stabilization of its structure in comparison with its non-acetylated form. It was also demonstrated using molecular dynamics that the replacement of acetylated α-tubulin in a complex with ATG8 by its non-acetylated form leads to a reduction in the interacting residues and, as a consequence, to a complete breakdown of the contact. Acetylation of α-tubulin at the Lys40 residue leads to the stabilization of both the protein itself and the complex under study with ATG8.

Key words: post-translational modifications, tubulin, ATG8, microtubules, in silico, molecular dynamics

Tsitologiya i Genetika 2021, vol. 55, no. 6, pp. 15-25

1. Laboratory of Bioinformatics and Structural Biology, Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Osipovskogo St., Kyiv, 04123, Ukraine
2. Taras Shevchenko National University of Kyiv, 64 Volodymyrska Street, Kyiv, 01601, Ukraine
3. Enamine Ltd., Chervonotkatska Street 78, Kyiv, 02094, Ukraine

E-mail: rayevsky85 gmail.com

Rayevsky A., Ozheredov D.S., Samofalova D., Ozheredov S.P., Karpov P.А., Blume Ya.B. The role of post-translational acetylation in the association of ATG8 autophagia protein with microtubules from plant cells, Tsitol Genet., 2021, vol. 55, no. 6, pp. 15-25.

In "Cytology and Genetics":
A. Rayevsky, D. S. Ozheredov, D. Samofalova, S. P. Ozheredov, P. A. Karpov & Ya. B. Blume The Role of Posttranslational Acetylation in the Association of Autophagy Protein ATG8 with Microtubules in Plant Cells, Cytol Genet., 2021, vol. 55, no. 6, pp. 510–518
DOI: 10.3103/S0095452721060128

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