Main page Contacts Preview papers   Contents   Themes Subscription Information to authors Editorial board Standard version

The level of expression NOS2 and NFKB1 Genes in esophageal alkaline burns and when melanin is administered

Chornenka N.M., Raetska Ya.B., Huet A.S., Beregova T.V., Savchuk O.M., Ostapchenko L.I.

SUMMARY. In this study, we have shown an increase in the expression of pro-inflammatory agents – Nos2 and Nfkb1 genes – in the blood and mucous membrane of the esophagus under conditions of the 2nd degree esophageal alkaline burn (EAB 2). It was found that under AEB 2, the activity of eNOS and iNOS increased both in the blood and mucous membrane of the esophagus. After the introduction of melanin, there was a decrease in the expression of Nos2 and Nfkb1 genes in the blood and esophageal tissues as compared with AEB 2. Also, there was a decrease in the activity of eNOS and iNOS as compared with AEB 2, indicating the anti-inflammatory properties of this compound and the possibility of using melanin as a substance that promotes faster healing of chemical burns of the esophagus and prevents post-burn complications.

Key words: alkaline burn of the esophagus, gene expression Nos2, Nfkb1, nitric oxide, melanin

Tsitologiya i Genetika 2021, vol. 55, no. 4, pp. 26-33

• Educational and Scientific Center «Institute of Biology and Medicine», Taras Shevchenko National University of Kyiv, Ukraine

E-mail: nata.chornenka24 gmail.com

Chornenka N.M., Raetska Ya.B., Huet A.S., Beregova T.V., Savchuk O.M., Ostapchenko L.I. The level of expression NOS2 and NFKB1 Genes in esophageal alkaline burns and when melanin is administered, Tsitol Genet., 2021, vol. 55, no. 4, pp. 26-33.

In "Cytology and Genetics":
N. M. Chornenka, Ya. B. Raetska, A. S. Huet, T. V. Beregova, O. M. Savchuk & L. I. Ostapchenko Expression Level of the Nos2 and Nfkb1 Genes in the Conditions of Esophageal Alkaline Burns and with the Administration of Melanin, Cytol Genet., 2021, vol. 55, no. 4, pp. 317–323
DOI: 10.3103/S0095452721040022

References

1. Abo El-Noor, M., Elgazzar, F., and Alshenawy, H., Role of inducible nitric oxide synthase and inter-leukin-6 expression in estimation of skin burn age and vitality, J. Forensic Leg. Med., 2017. https://doi.org/10.1016/j.jflm.2017.09.001

2. Ahmady El-Naggar, N. and El-Ewasy, S., Bioproduction, characterization, anticancer and antioxidant activities of extracellular melanin pigment produced by newly isolated microbial cell factories Streptomyces glaucescens NEAE-H, Sci. Rep., 2017. https://doi.org/10.1038/srep42129

3. Bartesaghi, S. and Radi, R., Fundamentals on the biochemistry of peroxynitrite and protein tyrosine nitration, Redox Biol., 2018. https://doi.org/10.1016/j.redox.2017.09.009

4. Basaran, U., Eskiocak, S., Altaner, S., et al., Inhibition of iNOS with S-methylisothiourea was impaired in wound healing in caustic esophageal burn, Int. J. Pediatr. Otorhinolaryngol., 2005. https://doi.org/10.1016/j.ijporl.2004.11.004

5. Boyde, T. and Rahmatullah, M., Optimization of conditions for the colorimetric determination of citrulline, using diacetyl monoxime, Anal. Biochem., 1980. https://doi.org/10.1016/0003-2697(80)90404-2

6. Carletti, G., Nervo, G., and Cattivelli, L., Flavonoids and melanins: a common strategy across two kingdoms, Int. J. Biol. Sci., 2014. https://doi.org/10.7150/ijbs.9672

7. Cartwright, T., Perkins, N., and Wilson, C., NFKB1: a suppressor of inflammation, ageing and cancer, FEBS J., 2016. https://doi.org/10.1111/febs.13627

8. Chomczynski, P. and Sacchi, N., Single-step method of RNA isolation by acid guanidiniumthiocyanate–phenol–chloroform extraction, Anal. Biochem., 1987. https://doi.org/10.1006/abio.1987.9999

9. Chornenka, N., Raetska, Y.A., Savchuk, O., et al., Effect of different doses of melanin in the blood protein changes in rats under alkaline esophageal burns, Res. J. Pharm. Biol. Chem. Sci., 2017, vol. 8, pp. 261–269.

10. Chyzhanska, N., Tsyryuk, O., and Beregova, T., The level of cortisol in the blood of rats before and after stress action against the background of melanin, Visn. Probl. Biol. Med., 2007, vol. 1, pp. 40–44.

11. Duan, H., Chai, J., Sheng, Z., et al., Effect of burn injury on apoptosis and expression of apoptosis-related genes/proteins in skeletal muscles of rats, Apoptosis: Int. J. Progr. Cell Death, 2009. https://doi.org/10.1007/s10495-008-0277-7

12. Eduardo, D., Rezende, B., Cortes, F., et al., Neuronal nitric oxide synthase in vascular physiology and diseases, Front. Physiol., 2016. https://doi.org/10.3389/fphys.2016.00206

13. Elobeid, A., Afaf Eldin, K., Abdelhalim, M., et al., Pharmacological properties of melanin and its function in health, Basic Clin. Pharmacol. Toxicol., 2017. https://doi.org/10.1111/bcpt.12748

14. Fistal, E., Kozinets, G., Samoilenko, G., et al., Combustiology. Textbook, Donetsk, 2005.

15. Kobayashi, Y., The regulatory role of nitric oxide in proinflammatory cytokine expression during the induction and resolution of inflammation, J. Leukoc. Biol., 2010. https://doi.org/10.1189/jlb.0310149

16. Kunwar, A., Adhikary, B., Jayakumar, S., et al., Melanin, a promising radioprotector: Mechanisms of actions in a mice model, Toxicol. Appl. Pharmacol., 2012. https://doi.org/10.1016/j.taap.2012.08.002

17. Mees, B., Wagner, S., Ninci, E., et al., Endothelial nitric oxide synthase activity is essential for vasodilation during blood flow recovery but not for arteriogenesis, Arterioscler., Thromb., Vasc. Biol., 2007. https://doi.org/10.1161/ATVBAHA.107.145375

18. Mitchell S, Vargas J, Hoffmann A. (2016) Signaling via the NF-κB System. Wiley Interdiscip Rev Syst Biol Med. https://doi.org/10.1002/wsbm.1331

19. Nakazawa H, Chang K, Shinozaki S et al. (2017) iNOS as a Driver of Inflammation and Apoptosis in Mouse Skeletal Muscle After Burn Injury: Possible Involvement of Sirt1 S-Nitrosylation-Mediated Acetylation of p65 NF-κB and p53. PLoS One. https://doi.org/10.1371/journal.pone.0170391

20. Nakazawa, H., Yamada, M., Tanaka, T., et al., Role of protein farnesylation in burn-induced metabolic derangements and insulin resistance in mouse skeletal muscle, PLoS One, 2015. https://doi.org/10.1371/journal.pone.0116633

21. Racca, S., Spaccamiglio, A., Esculapio, P., et al., Effects of swim stress and alpha-MSH acute pre-treatment on brain 5-HT transporter and corticosterone receptor, Pharmacol. Biochem. Behav., 2005. https://doi.org/10.1016/j.pbb.2005.06.014

22. Raetska, Ya., Ishchuk, T., Savchuk, O., et al., Experimental modeling of first-degree chemically-induced esophageal burns in rats, Med. Chem., 2013, vol. 15, no. 4, pp. 30–34.

23. Salih, E., Afaf, K., and Mohamed Anwar, K., Pharmacological properties of melanin and its function in health, Basic Clin. Pharmacol. Toxicol., 2017. https://doi.org/10.1111/bcpt.12748

24. Stanojcic, M., Abdullahi, A., Rehou, S., et al., Pathophysiological response to burn injury in adults, Ann. Surg., 2018. https://doi.org/10.1097/SLA.0000000000002097

25. Wang, Z., Li, S., Cao, Y., et al., Oxidative stress and carbonyl lesions in ulcerative colitis and associated colorectal cancer, Oxid. Med. Cell Longev., 2016. https://doi.org/10.1155/2016/9875298

26. Wigenstama, E., Elfsmarka, L., Buchtab, A., et al., Inhaled sulfur dioxide causes pulmonary and systemic inflammation leading to fibrotic respiratory disease in a rat model of chemical-induced lung injury, Toxicology, 2016. https://doi.org/10.1016/j.tox.2016.08.018

27. Zhou, X., Wang, H., Zhang, J., et al., Functional poly(e-caprolactone)/chitosan dressings with nitric oxide-releasing property improve wound healing, Acta Biomater., 2017. https://doi.org/10.1016/j.actbio.2017.03.011