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Phylogenetic analysis of the strain Bacillus subtilis IFBG MK-2 and production of riboflavin by its induced clones
SUMMARY. The aim of this work was to study the phylogenetic relationships of the new producer strain Bacillus subtilis IFBG ÌÊ-2 with existing strains and to increase the producer’s biosynthetic activity by mutagenesis. Se-quencing of the 16S rRNA of the IFBG MK-2 strain resulted in fragments of this gene with a total length of 1305 nucleotides. Phylogenetic analysis was performed and a dendrogram was constructed based on the nucleotide sequences of the 16S rRNA gene using the Neighbour joining method and the two-parameter Kimura model. It was shown that the similarity of sequenced fragments of 16S rRNA of strain IFBG MK-2 with fragments of deposited in GenBank sequence of this gene of a typical strain B. subtilis IAM 12118 was 99 %. According to the results of phylogenetic analysis, the IFBG strain MK-2 was identified as B. subtilis. Using the method of induced mutagenesis using UV radiation and sequential stepwise selection of clones, a mutant clone of B. subtilis IFBG MK-1A was obtained with a 50 % increase in the accumulation of riboflavin compared to the original strain.
Key words: Bacillus subtilis, riboflavin, mutagenesis, phylogeny, UV radiation, producer strain
E-mail: Shulga5 i.ua
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