Export citations UNIMARC BibTeX RIS
Studies on gamma rays induced cytomorphological variations and procurement of some induced novel mutants in Kalmegh [Andrographis paniculata (Burm F.) Nees.]
In order to increase genetic variability for improvement and better adaptation in the present days’ changing climate, present investigation has been done in Andrographis paniculata (Burm. F.) Nees., (also known as Kalmegh in Ayurveda), an ethnomedicinal wild plant with highly immuneboosting properties. For the purpose, healthy, dry and homogeneous seeds were treated with four doses of gamma rays from Co60 sources at the dose rate of 1.55 Gy per second(selected on the basis of LD50i.e. 25Gy (gray), 50Gy, 100Gy and 200Gy along with one set of nonirradiated seeds and sown in triplicates in a completely randomized block design (CRBD). Higher doses were detrimental hence not suggested for further mutation breeding experiments. Ho
Key words: Andrographis paniculata (Kalmegh), Chromosomal aberrations, Gamma rays, Genetic variation, Meiosis, Tricotyledon, Wild medicinal plants
E-mail: kshama.dwivedi gmail.com
1. Ahmad, S. and Yasmin, R., Effect of methyl parathion and trimillox on the mitosis of Allium cepa, Cytologia, 1992, vol. 57, pp. 155–160.
2. Aizen, M. and Harder, L., Expanding the limits of the pollen-limitation concept: effects of pollen quantity and quality, Ecology, 2007, vol. 88, pp. 271–281.
3. Ali, H., Ghori, Z., Sheikh, S., and Gul, A., Effects of gamma radiation on crop production, in Crop Production and Global Environmental Issues, Hakeem, K.R., Ed., Springer Int. Publ. Switzerland, 2015, pp. 27–78.
4. Benoy, G., Datta, A., Aninda, M., et al., An overview on Andrographis paniculata (Burm. F.) Nees, Int. J. Res. Ayurveda Pharm., 2012, vol. 6, pp. 752–760.
5. Chatterjee, A., Shukla, S., Mishra, B., et al., Induction of variability through mutagenesis in opium poppy (Papaver somniferum L.), Turk. J. Bot., 2010, vol. 36, pp. 1–11.
6. Chen, D.C., Tang, Z.S., and Yang, J., Changes in cotyledons of Impatiens balsamina in third generation (SP3) induced by space flight, J. Trop. Subtrop. Bot., 2006, vol. 14, pp. 202–206.
7. Chopra, V. and Sharma, R., Induced mutations in crop improvement, in Genetic Manipulation for Crop Improvement, New Delhi: Oxford and IBH Publ. Co., 1985, pp. 23–48.
8. Dafni, A. and Firmage, D., Pollen viability and longevity: practical, ecological and evolutionary implications, Plant Syst. Evol., 2000, vol. 222, pp. 113–132.
9. Dixit, V., Prabha, R., and Chaudhary, B., Effects of EMS and SA on meiotic cells and thymoquinone content of Nigella sativa L. cultivars, Caryologia, 2013, vol. 66, pp. 178–185.
10. Foyer, C. and Harbinson, J., Oxygen metabolism and the regulation of photosynthetic electron transport, in Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants, Foyer, C.H. and Mullineaux, P.M., Eds., CRC Press, 1994, pp. 1–42.
11. Gustafsson, R., Productive mutations induced in barley by ionizing radiations and chemical mutagens, Heriditas, 1963, vol. 17, pp. 211–262.
12. Hanafy, R. and Akladious, A., Physiological and molecular studies on the effect of gamma radiation in fenugreek (Trigonella foenum-graecum L.) plants, J. Genet. Eng. Biotechnol., 2018, vol. 16, pp. 683–692.
13. Hu, J., Miller, J.F., Chen, J., and Vick, B.A., Preliminary observations on spontaneous tricotyledonous mutant, in Proc. 27th Sunflower Research Forum, January 12–13, 2005.
14. Hu, J., Miller, J.F., and Vick, B.A., Registration of a tricotyledon sunflower genetic stock, Crop Sci., 2006, vol. 46, p. 2734.
15. Fargo, N.D., Joselin, J., and Jeeva, S., Andrograhis paniculata: a review of its traditional uses, phytochemistry and pharmacology, Med. Aromat. Plants, 2014, vol. 14, pp. 1–15.
16. Karpate, R. and Choudhary, A., Induced mutation in Linum usitatissimum L., J. Cytol. Genet., 1997, vol. 32, pp. 41–48.
17. Katiyar, R., Radiocytogenetical studies on Capsicum: meiotic abnormalities, Cytologia, 1978, vol. 43, pp. 415–421.
18. Kim, J., Baek, M., Lee, Y., Lee, H., and Park, Y., Stimulating effect of low dose gamma-ray radiation on the growth and physiological activities of Chinese cabbage cultivars, in Proceedings of the 12th International Congress on Photosynthesis, Brisbane, 2004.
19. Kleinhofs, A., Owais, W., and Nilan, R., Azide, Mutat. Res., 1978, vol. 55, pp. 165–195.
20. Kumar, G. and Yadav, R., EMS induced genomic disorders in sesame (Sesamum indicum L.), Rom. J. Biol.–Plant Biol., 2010, vol. 55, pp. 97–104.
21. Lattoo, S., Khan, S., Dhar, A., Choudhary, D., et al., Genetics and mechanism of induced male sterility in Andrographis paniculata (Burm. f.) Nees and its significance, Curr. Sci., 2006, vol. 91, pp. 515–519.
22. Liang, Y., Li, Z., Shen, W., and Huang, F., Cotyledon diversity and seedling characteristics of Michelia macclurei dandy from natural populations, Bangladesh J. Bot., 2018, vol. 47, pp. 17–23.
23. Lichtenthaler, H.K. and Wellburn, A.R., Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents, Biochem. Soc. Transact., 1983, vol. 11, pp. 591–592.
24. Liu, B., De Storme, N., and Geelen, D., Cold-induced male meiotic restitution in Arabidopsis thaliana is not mediated by GA-DELLA signaling bing, Front. Plant Sci., 2018, vol. 9, p. 91.
25. Luckey, T., Hormesis with Ionizing Radiation, Boca Raton: CRC press, 1980.
26. Majeed, A., Khan, A., Ahmad, H., and Muhammad, Z., Gamma irradiation effects on some growth parameters of Lepidium sativum L., ARPN J. Agric. Biol. Sci., 2010, vol. 5, pp. 39–42.
27. Morgan, W. and Sowa, M., Effects of ionizing radiation in non-irradiated cells, Proc. Natl. Acad. Sci. U. S. A., 2005, vol. 102, pp. 14127–14128.
28. Pollard, E., Ionizing radiation: effect on genetic transcription, Science, 1964, vol. 146, pp. 927–929.
29. Porter, L.A. and Lee, J.M., Alpha-, beta-, and gamma-tubulin polymerization in response to DNA damage, Exp. Cell Res., 2001, vol. 270, pp. 151–158. https://doi.org/10.1006/excr.2001.5322
30. Preussa, S. and Britta, A., A DNA-damage-induced cell cycle checkpoint in Arabidopsis, Genetics, 2003, vol. 64, pp. 323–334.
31. Roy, S.K. and Datta, P.C., Chromosomal biotypes of Andrographis paniculata in India and Bangladesh, Cytologia, 1988, vol. 53, pp. 369–378.
32. Smertenko, A., Draber, P., Viklicky, V., and Opatrny, Z., Heat stress affects the organization of microtubules and cell division in Nicotiana tabacum cells, Plant Cell Environ., 1997, vol. 20, pp. 1534–1542.
33. Song, M., Wei, Q., Wang, J., et al., Fine mapping of CsVYL, conferring virescent leaf through the regulation of chloroplast development in cucumber, Front. Plant Sci., 2018, vol. 9, pp. 1–12.
34. Srivastava, A., Misra, H., Verma, R., and Gupta, M., Chemical fingerprinting of Andrographis paniculata using HPLC, HPTLC and densitometry, Phytochem. Anal., 2004, vol. 15, pp. 280–285.
35. Stern, D.B., Hanson, M.R., and Barkan, A., Genetics and genomics of chloroplast biogenesis: maize as a model system, Trends Plants Sci., 2004, vol. 9, pp. 293–301. https://doi.org/10.1016/j.tplants.2004.04.001
|Coded & Designed by Volodymyr Duplij||Modified 01.12.23|