ISSN 0564-3783  
Main page
Contacts
Preview papers  
Contents  
Themes
Subscription
Information to authors
Editorial board
Standard version



In Ukrainian

Export citations   UNIMARC   BibTeX   RIS


Genetic polymorphism and variability of Anacamptis morio s. l. (Orchidaceae Juss.) population in Ukraine

Gaponenko M.B., Blum O.B., Kashevarov G.P.

 




SUMMARY. The results of the population genetic and taxonomic analysis of the critical species of Orchids, viz. Anacamptis morio s. l. in the flora of Ukraine are presented. The genetic analysis was carried out using PCR analysis (RAPD-, ISSR amplification) and DNA sequencing (matK, atpF-atpH, rbcL) and genetic differentiation of populations of these plants from Transcarpathian Ukraine (Zakarpattia), Ukrainian Polissia and Crimea. A significant level of genetic difference between the Crimean populations and populations from Zakarpattia and Ukrainian Polissia was established, as well as a high level of genetic similarity between all of the studied populations of A. morio subsp. caucasica from Crimea. The highest level of genetic polymorphism of the studied populations was detected by the RAPD-markers OPA-11, OPP-10, OPA-18, UBC-297, as well as the ISSR-marker (AG)8TC. The main indices of genetic polymorphism of the five A. morio populations were as follows: the share of polymorphic loci (P) 40,074,8 %; the effective number of alleles (ne) 1,2761,479; the gene diversity (h) 0,1560,276; the Shannon information index (I) 0,2290,408; the genetic distances between populations according to Nei (DN) 0,0470,325. For a more complete genetic characterization of populations, the following characteristics were also determined: expected total gene diversity (Ht); intrapopulation gene diversity (Hs); coefficient of gene differentiation of populations (Gst) as well as the value of gene flow (Nm). Based on the results of molecular genetic studies and the analysis of morphological and biological data in the flora of Ukraine, it is proposed to delimitate the taxa as follows: A. morio subsp. morio (L.) R.M. Bateman, Pridgeon et M.W. Chase, which grows in the Ukrainian Carpathians and the Polissia as well as A. morio subsp. caucasica (K. Koch) H. Kretzschmar, Eccarius et H. Dietr. distributed in the Crimea and the Northern Black Sea region on the territory of Ukraine.

Key words: Orchidaceae, Anacamptis morio, rare plants, genetic polymorphism, PCR analysis, RAPD-, ISSR-markers, sequencing, matK, atpF-atpH, rbcL, Ukraine

Tsitologiya i Genetika 2021, vol. 55, no. 4, pp. 3-15

  • M.M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine, Timiryazevska str. 1., 010141, Kyiv, Ukraine

E-mail: gaponenko nbg.kiev.ua

Gaponenko M.B., Blum O.B., Kashevarov G.P. Genetic polymorphism and variability of Anacamptis morio s. l. (Orchidaceae Juss.) population in Ukraine, Tsitol Genet., 2021, vol. 55, no. 4, pp. 3-15.

In "Cytology and Genetics":
M. B. Gaponenko, O. B. Blum & G. P. Kashevarov Genetic Polymorphism and Variability of the Anacamptis morio s.l. (Orchidaceae Juss.) Population in Ukraine, Cytol Genet., 2021, vol. 55, no. 4, pp. 299308
DOI: 10.3103/S0095452721040046


References

1. Averyanov, L.V., Orchidaceae Juss., in Caucasian Flora Conspectus, Tahktadjan, A.L., Ed., St. Petersburg: St. Petersburg Univ. Press, 2006, pp. 84101.

2. Bateman, R.M., Hollingsworth, P.M., Preston, J., Luo, Y.-B., Pridgeon, A.M., and Chase, M.W., Molecular phylogenetics and evolution of Orchidinae and selected Habenariinae (Orchidaceae), Bot. J. Linn. Soc., 2003, vol. 142, no. 1, pp. 140. https://doi.org/10.1046/j.1095-8339.2003.00157.x

3. Bateman, R.M., Murphy, A.R.M., Hollingsworth, P.M., Hart, M.L., Denholm, I., and Rudall, P.J., Molecular and morphological phylogenetics of the digitate-tubered clade within subtribe Orchidinae s.s. (Orchidaceae: Orchideae), Kew Bull., 2018, vol. 73, p. 54. https://doi.org/10.1007/s12225-018-9782-1

4. Bateman, R.M., Pridgeon, A.M., and Chase, M.W., Phylogenetics of subtribe Orchidinae (Orchidoideae, Orchidaceae) based on nuclear ITS sequences. 2. Infragene-ric relationships and reclassification to achieve monophyly of Orchis sensu strict, Lindleyana, 1997, vol. 12, no. 3, pp. 113141.

5. Bordzilovskyi, E.I., Orchidaceaem, in Flora UkrRSR, Kotov, M.I. and Barbarych, A.I., Eds., Kyiv: Akad. Nauk UkrRSR, 1950, vol. 3, pp. 312405.

6. Chase, M.W., Cameron, K.M., Freudenstein, J.V., Pridgeon, A.M., Salazar, G., van den Berg, C., and Schuiteman, A., An updated classification of Orchidaceae, Bot. J. Linn. Soc., 2015, vol. 177, no. 2, pp. 151174.

7. Christenhusz, M.J.M. and Byng, J.W., The number of known plants species in the world and its annual increase, Phytotaxa, 2016, vol. 261, no. 3, pp. 201217. https://doi.org/10.11646/phytotaxa.261.3.1

8. Fateryga, A.V., New checklist of orchids (Orchidaceae) in the flora of the Crimea, Ekosistemy, 2019, vol. 17, pp. 3843.

9. Fateryga, A.V., Efimov, P.G., and Svirin, S.A., Orchids of the Crimean Peninsula, Simferopol: Arial, 2019, p. 224.

10. Fateryga, A.V. and Kreutz, C.A.J., Checklist of the orchids of the Crimea (Orchidaceae), J. Eur. Orch., 2014, vol. 46, no. 2, pp. 407436.

11. Govaerts, R., Bernet, P., Kratochvil, K., Gerlach, G., Carr, G., Alrich, P., Pridgeon, A.M., Pfahl, J., Campacci, M.A., Holland Baptista, D., Tigges, H., Shaw, J., Cribb, P., George, A., Kreutz, K., and Wood, J., World Checklist of Orchidaceae, Kew: Royal Botanic Gardens, 20052020. http://apps.kew.org/wcsp/. Accessed August 28, 2020.

12. Grossgeym, A.A., Analysis of the Flora of the Caucasus, Baku: Azerbaijan Publishing House Branch of the Acad. of Sci. of the USSR, 1936.

13. Kimura, M. and Crow, J.F., The number of alleles that can be maintained in a finite population, Genetics, 1964, vol. 49, no. 4, pp. 725538.

14. Koneva, A.Yu., Genetic estimation of snail Lymnaea stagnalis populations from regions with different anthropogenic loads as a first step in genetic monitoring, Ecol. Genet., 2012, vol. 10, no. 2, pp. 2131. https://doi.org/10.17816/ecogen10221-31

15. Kretzschmar, H., Eccarius, W., and Dietrich, H., The Orchid Genera Anacamptis, Orchis, Neotinea, Brugel, 2007.

16. Kuropatkin, V.V. and Efimov, P.G., A review of the genera Anacamptis, Neotinea and Orchis s. str. (Orchidaceae) in the flora of Russia and adjacent countries, with considerations of subdivision of Orchis s.l., Bot. Zh., 2014, vol. 99, no. 5, pp. 555593: https://doi.org/10.1134/S1234567814050061

17. Lewontin, R.C., The apportionment of human diversity, in Evolutionary Biology, Dobzhansky, T., Hecht, M.K., and Steere, W.C., Eds., New York, NY: Springer, 1972, pp. 381398. https://doi.org/10.1007/978-1-4684-9063-3_14

18. Matveeva, T.V., Pavlova, O.A., Bogomaz, D.I., Demkovich, A.E., and Lutova, L.A., Molecular markers for plant species identification and phylogenetics, Ecol. Genet., 2011, vol. 9, no. 1, pp. 3243. https://doi.org/10.17816/ecogen9132-43

19. Mikhailova, Yu.V., Machs, E.M., Rodionov, A.V., and Razzhivin, V.Yu., in Proceedings of the All-Russian Conference Fundamental and Applied Problems of Botany at the Beginning of the 21st Century (Petrozavodsk, September 2227, 2008), Petrozavodsk: Karel. Sci. Center Russ. Acad. Sci., 2008, part 3, pp. 5255.

20. Morin, C., Breuil, C., and Bernier, L., Genetic variability and structure of Canadian populations of the sapstain fungus Ceratocystis resinifera, Phytopathology, 2004, vol. 94, no. 12, pp. 13231330.

21. Nei, M., Genetic distance between populations, Am. Nat., 1972, vol. 106, pp. 283292.

22. Nei, M., Analysis of gene diversity in subdivided populations, Proc. Natl. Acad. Sci. U. S. A., 1973, vol. 70, no. 12, pp. 33213333. https://doi.org/10.1073/pnas.70.12.3321

23. Nei, M., F-statistics and analysis of gene diversity in subdivided populations, Ann. Hum. Genet., 1977, vol. 41, no. 2, pp. 225233. https://doi.org/10.1111/j.1469-1809.1977.tb01918.x

24. Nei, M., Estimation of average heterozygosity and genetic distance from a small number of individuals, Genetics, 1978, vol. 89, pp. 583590.

25. Nei, M., Molecular Evolutionary Genetics, Columbia University Press, 1987. https://doi.org/10. 7312/nei-92038

26. Nei, M. and Chesser, R.K., Estimation of fixation indices and gene diversities, Ann. Hum. Genet., 1983, vol. 47, pp. 253259. https://doi.org/10.1111/j.1469-809.1983.tb00993.x

27. Nevski, S.A., Family Orchidaceae Lindl, in Flora of USSR, Komarov, V.L., Ed., Leningrad: Acad. Sci. USSR, 1935, vol. 4. pp. 589730.

28. Protopopova, V.V., Orchidaceae, in Determinant of Higher Plants of Ukraine, Kyiv: Naukova Dumka, 1987, pp. 405412.

29. Red Data Book of Ukraine. Plant Kingdom, Didukh, Ya.P., Ed., Kyiv: Globalconsulting, 2009.

30. Rogers, S.O. and Bendich, A.J., Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues, Plant Mol. Biol., 1985, vol. 5, pp. 6976.

31. Rossi, W., Corrias, B., Arduino, P., Cianchi, R., and Bullini, L., Gene variation and gene flow in Orchis morio (Orchidaceae) from Italy, Plant Syst. Evol., 1992, vol. 179, pp. 4348. https://doi.org/10.1007/BF00938018

32. Smolyaninova, L.A., Orchidaceae Juss., in Flora of the European Part of USSR, Fedorov, A.A. and Egorova, T.V., Eds., Leningrad: Nauka,1976, vol. 2, pp. 1059.

33. Sobko, V.G., Orchids of Ukraine, Kyiv: Naukova Dumka, 1989.

34. Vázquez, F.M., Blanco, J., Garcia, D., Márquez, F., and Guerra, M.J., Review of Anacamptis sect. Morianthus taxa from SW Iberian Peninsula, J. Eur. Orch., 2015, vol. 47, nos. 24, pp. 338364.

35. Vakhrameeva, M.G., Denisova, L.V., Nikitina, S.V., and Samsonov, S.K., Orchids in Our Country, Moscow: Science, 1991.

36. Wulff, E.W., Flora Taurica, Leningrad: Nikitskiy Botanical Garden Press, 1930, vol. 1, no. 3.

37. Yeh, F.C., Yang, R., and Boyle, T., POPGENE Version 1.32: Microsoft Windows-Based Freeware for Population Genetic Analysis, Edmonton, Canada: University of Alberta, 1999.

38. Zavadsky, K.M., Species and Speciation, Leningrad: Science, 1967.

Copyright© ICBGE 2002-2023 Coded & Designed by Volodymyr Duplij Modified 01.12.23