ISSN 0564-3783  
Main page
Preview papers  
Information to authors
Editorial board
Standard version

In Ukrainian

Export citations   UNIMARC   BibTeX   RIS

Identification of genotypes with recombinant arm 1RS in bread wheat segregating F5 populations from crosses between carriers of 1BL.1RS AND 1AL.1RS

Kozub N.O., Sozinov I.O., Bidnyk H.Ya., Demianova N.O., Sozinova O.I., Karelov A.V., Borzykh O.I., Blume Ya.


SUMMARY. Wheat-rye translocations involving arm 1RS are widespread introgressions among commercial bread wheat cultivars: 1BL.1RS from the rye Petkus (as in the cultivar Kavkaz) and 1AL.1RS from the rye Insave (as in Amigo). Genotypes with recombinant arm 1RS involved in the translocation can carry new combinations of resistance genes in the chromosome region flanked by the loci Sec-1 (Gli-R1) and Sec-N, which is located distal to Sec-1 at a distance of 1020 cM. The aim of this investigation was to study the frequency of genotypes with recombinant 1RS arms involved in wheat-rye translocations in segregating F5 populations from crossing bread wheat carriers of translocations 1BL.1RS and 1AL.1RS. Samples of F5 seeds from the crosses Myronivska 67 (M67) × Kolumbiia, Kolumbiia × M67, B-16 × Smuhlianka, and Smuhlyanka × B-16 served as the material for the investigation. The cultivar M67 and the line B-16 carry 1BL.1RS as in the cultivar Kavkaz; Smuhlianka and Kolumbiia carry 1AL.1RS as in the cultivar Amigo. Electrophoresis of alcoholsoluble grain proteins and identification of alleles at the Gli-A1/Gli-R1, Gli-B1/Gli-R1 loci were used to identify translocations involving 1RS and their position (on 1AL or 1BL). Sec-N alleles were identified by SDS electrophoresis. The presence of recombination products between the 1RS arms was determined based on the change of the chromosomal position of the respective allele at Gli-R1 (Sec-1) and Sec-N. In the segregating populations, there were five of six theoretically possible variants of genotypes with recombinant arm 1RS with respect to the secalin loci. The total frequency of genotypes with the simultaneous presence of two translocations ranged from 3.3 to 18.0 %, and the total frequency of genotypes with one identified recombinant arm 1RS was 5.730.5 %. The highest frequency of genotypes with recombinant arm 1RS was found in the population M67 × Kolumbiia.

Tsitologiya i Genetika 2022, vol. 56, no. 5, pp. 52-60

  1. Institute of Plant Protection of the National Academy of Agrarian Sciences of Ukraine, 03022, 33, Vasylkivska St., Kyiv
  2. Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine, 04123, 2 a, Osypovskogo St., Kyiv

E-mail: natalkozub

Kozub N.O., Sozinov I.O., Bidnyk H.Ya., Demianova N.O., Sozinova O.I., Karelov A.V., Borzykh O.I., Blume Ya. Identification of genotypes with recombinant arm 1RS in bread wheat segregating F5 populations from crosses between carriers of 1BL.1RS AND 1AL.1RS, Tsitol Genet., 2022, vol. 56, no. 5, pp. 52-60.

In "Cytology and Genetics":
N. O. Kozub, I. O. Sozinov, H. Ya. Bidnyk, N. O. Demianova, O. I. Sozinova, A. V. Karelov, O. I. Borzykh & Ya. B. Blume Identification of Genotypes with Recombinant Arm 1RS In Bread Wheat Segregating F5 Populations from Crosses Between Carriers of 1BL.1RS and 1AL.1RS, Cytol Genet., 2022, vol. 56, no. 5, pp. 441448
DOI: 10.3103/S0095452722050061


Bhattacharya, S., Deadly new wheat disease threatens Europes crops, Nature, 2017, vol. 542, pp. 145146.

Graybosch, R., Bai, G., Amand, P.S., et al., Persistence of rye (Secale cereale L.) chromosome arm 1RS in wheat (Triticum aestivum L.) breeding programs of the Great Plains of North America, Genet. Resour. Crop Evol., 2019, vol. 66, pp. 941950.

Hsam, S.L.K., Mohler, V., Hartl, L., et al., Mapping of powdery mildew and leaf rust resistance genes on the wheat-rye translocated chromosome T1BL1RS using molecular and biochemical markers, Plant Breed., 2000, vol. 119, no. 1, pp. 8789.

Kozub, N.A., Sozinov, I.A., Sobko, T.A., et al., Variation at storage protein loci in winter common wheat cultivars of the Central Forest-Steppe of Ukraine, Cytol. Genet., 2009, vol. 43, no. 1, pp. 5562.

Kozub, N.A., Motsnyi, I.I., Sozinov, I.A., et al., Mapping a new secalin locus on the rye 1RS arm, Cytol. Genet., 2014, vol. 48, no. 4, pp. 203207.

Kozub, N., Sozinov, I., Karelov, A., et al., Studying recombination between the 1RS arms from the rye Petkus and Insave involved in the 1BL.1RS and 1AL.1RS translocations using storage protein loci as genetic markers, Cytol. Genet., 2018, vol. 52, no. 6, pp. 440447.

Kozub, N.O., Sozinov, I.O., Chaika, V.M., et al., Changes in allele frequencies at storage proteins of winter common wheat under climate change, Cytol. Genet., 2020, vol. 54, pp. 305317.

Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 1970, vol. 227, no. 5259, pp. 680685.

Li, G., Wang, L., Yang, J., et al., A high-quality genome assembly highlights rye genomic characteristics and agronomically important genes, Nat. Genet., 2021, vol. 53, no. 4, pp. 574584.

Liu, S., Rudd, J.C., Bai, G., et al., Molecular markers linked to important genes in hard winter wheat, Crop Sci., 2014, vol. 54, pp. 13041321.

Lukashewski, A.J., Manipulation of the 1RS.1BL translocation in wheat by induced homoeologous recombination, Crop Sci., 2000, vol. 40, no. 1, pp. 216225.

Mago, R., Zhang, P., Vautrin, S., et al., Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines, Theor. Appl. Genet., 2002, vol.104, no. 17, pp. 13171324.

Mago, R., Miah, H., Lawrence, G.J., et al., High-resolution mapping and mutation analysis separate the rust resistance genes Sr31, Lr26 and Yr9 on the short arm of rye chromosome 1, Theor. Appl. Genet., 2005, vol. 112, no. 1, pp. 4150.

Mago, R., Zhang, P., Vautrin, S., et al., The wheat Sr50 gene reveals rich diversity at a cereal disease resistance locus, Nat. Plants, 2015, vol. 1, art. ID 15186.

Mater, Y., Baenziger, S., Gill, K., et al., Linkage mapping of powdery mildew and greenbug resistance genes on recombinant 1RS from Amigo and Kavkaz wheat-rye translocations on chromosome 1RS.1AL, Genome, 2004, vol. 47, no. 2, pp. 292298.

McIntosh, R.A., Catalogue of Gene Symbols. Gene Catalogue, 2013. genes/download.jspMacGene.

Metakovsky, E., Melnik, V., Rodriguez-Quijano, M., et al., A catalog of gliadin alleles: Polymorphism of 20th-century common wheat germplasm, Crop J., 2018, vol. 6, no. 6, pp. 628641.

Olivera Firpo, P.D., Newcomb, M., Flath, K., et al., Characterization of Puccinia graminis f. sp. tritici isolates derived from an unusual wheat stem rust outbreak in Germany in 2013, Plant Pathol., 2017, vol. 66, no. 8, pp.12581266.

Olivera, P.D., Sikharulidze, Z., Dumbadze, R., et al., Presence of a sexual population of Puccinia graminis f. sp. tritici in Georgia provides a hotspot for genotypic and phenotypic diversity, Phytopathology, 2019, vol. 109, no. 12, pp. 21522160.

Olivera, P.D., Villegas, D., Cantero-Martinez, C., et al., A unique race of the wheat stem rust pathogen with virulence on Sr31 identified in Spain and reaction of wheat and durum cultivars to this race, Plant Pathol., 2022, vol. 71, no. 4, pp. 873889.

Patpour, M., Justesen, A.F., Tecle, A.W., et al., First report of race TTRTF of wheat stem rust (Puccinia graminis f. sp. tritici) in Eritrea, Plant Dis., vol. 104, no. 3, art. ID 973.

Pretorius, Z.A., Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis f. sp. tritici, Plant Dis., 2000, vol. 84, no. 2, art. ID 203.

Purnhauser, L., Bona, L., and Lang, L., Occurrence of 1BL.1RS wheat-rye chromosome translocation and of Sr36/Pm6 resistance gene cluster in wheat cultivars registered in Hungary, Euphytica, 2011, vol. 179, pp. 287295.

Rabinovich, S.V., Importance of wheat-rye translocations for breeding modern cultivars of Triticum aestivum L., Euphytica, 1998, vol. 100, nos. 13, pp. 323340.

Rogowsky, P.M., Guidet, F.L., Langridge, P., et al., Isolation and characterization of wheat-rye recombinants involving chromosome arm 1DS of wheat, Theor. Appl. Genet., 1991, vol. 82, no. 5, pp. 537544.

Ru, Z., Juhasz, A., Li, D., et al., 1RS.1BL molecular resolution provides novel contributions to wheat improvement, bioRxiv, 2020.

Sasek, A. and Bartos, P., Gliadinova spectra odrud psenice s 1B/1R transolkaci nebo sustitui, Sb. UVTIZ, Genet. Slechteni, 1980, vol. 16, no. 4, pp. 243251.

Schlegel, R., Current list of wheats with rye and alien introgression, 2016, Version 05-16, pp. 118.

Sharma, S., Bhat, P.R., Ehdaie, B., et al., Integrated genetic map and genetic analysis of a region associated with root traits on the short arm of rye chromosome 1 in bread wheat, Theor. Appl. Genet., 2009, vol. 119, no. 5, pp. 783793.

Singh, N.K., Shepherd, K.W., and McIntosh, R.A., Linkage mapping of genes for resistance to leaf, stem and stripe rusts and ω-secalins on the short arm of rye chromosome 1R, Theor. Appl. Genet., 1990, vol. 80, no. 5, pp. 609616.

Singh, R.P., Hodson, D.P., Jin, Y., et al., Emergence and spread of new races of wheat stem rust fungus: continued threat to food security and prospects of genetic control, Phytopathology, 2015, vol. 105, no. 7, pp. 872884.

Singh, S.P., Hurni, S., Ruinelli, M., et al., Evolutionary divergence of the rye Pm17 and Pm8 resistance genes reveals ancient diversity, Plant Mol. Biol., 2018, vol. 98, pp. 249260.

Sozinov, A.A. and Poperelya, F.A., Gliadin electrophoresis as a method of wheat identification of wheats in which chromosome 1B is completely or partially replaced by rye chromosome 1R, Dokl. VASKhNIL, 1977, vol. 2, pp. 24.

Tabibzadeh, N., Karimzadeh, G., and Naghavi, M.R., Distribution of 1AL.1RS and 1BL.1RS wheat-rye translocations in Iranian wheat, using PCR based markers and SDS-PAGE, Cereal Res. Commun., 2013, vol. 41, pp.458467.

Tesfaye, T., Chala, A., Shikur, E., et al., First report of TTRTF race of wheat stem rust, Puccinia graminis f. sp. tritici, in Ethiopia, Plant Dis., 2020, vol. 104, pp. 293293.

Zhang, L., Liu, D., Guo, X., et al., Investigation of genetic diversity and population structure of common wheat cultivars in northern China using DArT markers, BMC Genet., 2011, vol. 12, art. ID 42.

Copyright© ICBGE 2002-2023 Coded & Designed by Volodymyr Duplij Modified 30.05.23