Цитологія і генетика 2021, том 55, № 5, 3-12
Cytology and Genetics 2021, том 55, № 5, 405–413, doi: https://www.doi.org/10.3103/S0095452721050108

Молекулярна організація та поліморфізм 5S рДНК карпатської бджоли

Рошка Н.М., Череватов О.В., Волков Р.А.

  • Чернівецький національний університет імені Юрія Федьковича вул. Коцюбинського, 2, Чернівці, 58012, Україна

Природний ареал медоносної бджоли, Apis mellifera L. охоплює Європу, Західну Азію та Африку. Адаптація до локальних умов існування призвела до формування чисельних підвидів та екотипів медоносної бджоли, які являють собою зручну модель для дослідження мікроеволюції комах. Ділянки геному, які кодують 5S рРНК (5S рДНК), є популярним інструментом для вивчення молекулярної еволюції та філогенії близькоспоріднених форм тварин і рослин. У цій статті ми наводимо результати аналізу поліморфізму 5S рДНК двох селекційних рас – Рахівська та Говерла – Карпатської породи медоносної бджоли, яка представляє місцевий екотип підвиду A. m. carnica, та порівнюємо їх із даними, наявними у базі даних Genbank для цього підвиду та азійського виду A. cerana. Встановлено, що в геномі A. m. carnica присутні щонайменше два класи 5S рДНК, кожен з яких містить декілька структурних варіантів. Геноми двох досліджених рас Карпатської породи та наявний у Genbank зразок A. m. carnica відрізняються за наборами таких варіантів, тоді як повторювані ділянки 5S рДНК A. cerana є ідентичними в межах геному. Отримані результати свідчать про високий внутрішньо- та міжгеномний поліморфізм 5S рДНК у A. m. carnica.

Ключові слова: карпатська бджола, міжгенний спейсер 5S рДНК, молекулярні маркери, повторювані послідовності, Apis mellifera, Apidae

Цитологія і генетика
2021, том 55, № 5, 3-12

Current Issue
Cytology and Genetics
2021, том 55, № 5, 405–413,
doi: 10.3103/S0095452721050108

Повний текст та додаткові матеріали

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