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The luminescence of blood leukocytes, stained with the fluorochrome acridine orange, from surgical patients in the dynamics of emotional, anesthesiological and surgical stress

Frolov A.K., Shifrin G.A., Fedotov E.R., Gritsaenko Iu.M.

 




Luminescence of blood leukocytes of surgical patients during treatment was studied using orange acridine. Luminescence intensity at 640 nm wave length (I640) was the most informative. It characterized amount of single-stranded nucleic acids, synthesized in cell due to previous activation of protein-synthesizing system and hence cell functional activity, while in lymphocytes these changes exceeded by duration and amplitude those in neutrophils. During premedication (emotional stress) I640 was minimal, during anesthetization it developed, and sharply raised during traumatic period of surgery and after it, as reflection of immune system participation in reparative regeneration.

Tsitologiya i Genetika 1999, vol. 33, no. 6, pp. 14-19



Frolov A.K., Shifrin G.A., Fedotov E.R., Gritsaenko Iu.M. The luminescence of blood leukocytes, stained with the fluorochrome acridine orange, from surgical patients in the dynamics of emotional, anesthesiological and surgical stress, Tsitol Genet., 1999, vol. 33, no. 6, pp. 14-19.




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