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Molecular genetic evaluation of the ukrainian flax variaties homogeneity based on actin gene intronpolymorphism and microsatellite loci

Postovoitova А.S., Yotka O.Yu., Рirko Ya.V., Blume Ya.B.

 




SUMMARY. The polymorphism of introns length of actin genes and microsatellite loci have been investigated for estimation of genetic homogeneity of varieties of flax of Ukrainian selection. It is established that the vast majority of varieties of flax are genetically heterogeneous. The varieties of Esman, Seversky and Glazur breeding of the Institute of Bast Crops of the NAAS of Ukraine have been genetically homogeneous by the results of analysis of the introns length polymorphism of actin genes and microsatellite markers. It has been shown that the intron length of polymorphism of actin genes is not less informative marker system for genetic profiling in comparison with popular SSR markers. Data were obtained confirming the expediency of further simultaneous use of the mentioned DNA marker systems to evaluate the genetic diversity of varieties of flax.

Key words: DNA marker, actin, intron length polymorphism, SSR (simple sequence repeats), intraspecies polymorphism, flax (Linum usitatissimum L.)

Tsitologiya i Genetika 2018, vol. 52, no. 6, pp. 71-85

E-mail: nastya.postovoytova gmail.com, flax­dslk ukr.net, yarvp1 gmail.com, cellbio cellbio.freenet.viaduk.net

Postovoitova А.S., Yotka O.Yu., Рirko Ya.V., Blume Ya.B. Molecular genetic evaluation of the ukrainian flax variaties homogeneity based on actin gene intronpolymorphism and microsatellite loci, Tsitol Genet., 2018, vol. 52, no. 6, pp. 71-85.

In "Cytology and Genetics":
A. S. Postovoitova, O. Yu. Yotka, Ya. V. Pirko, Ya. B. Blume Molecular Genetic Evaluation of Ukrainian Flax Cultivar Homogeneity Based on Intron Length Polymorphism of Actin Genes and Microsatellite Loci, Cytol Genet., 2018, vol. 52, no. 6, pp. 448–460
DOI: 10.3103/S0095452718060099


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