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Arabidopsis root morphology and its correlation with cortical microtubule rearrangements caused by specific inhibition of protein kinases CK1

Karpov P.A., Sheremet Ya.A., Blume Ya.B., Yemets A.I.

 




SUMMARY. The members of the casein kinase 1 (CK1) subfamily are distinguished by strong interspecific conservatism and huge number of phosphorylation substrates. Recent data demonstrate evidence of direct CK1-dependent phosphorylation of tubulin. Consequently, CK1 protein kinases can contribute in the tubulin code and functional specialization of microtubules (MT). In this paper, we present new data on the plant tubulin cytoskeleton response provoked by treatment with D4476 specific inhibitor of CK1. It was found that D4476 demonstrate a strong and dose-dependent effect on the growth and morphology of primary roots in Arabidopsis thaliana seed-lings. Subsequent experiments, on plants expressing a MT-associated fluorescent marker (GFP-Map4), have shown a pronounced correlation between morphological reac-tions and cortical rearrangements of microtubules caused by D4476-dependent inhibition of protein kinases CK1.

Key words: casein kinase 1, microtubules, phosphorylation, inhibitor, D4476, Arabidopsis

Tsitologiya i Genetika 2019, vol. 53, no. 6, pp. 3-14

E-mail: karpov nas.gov.ua

Karpov P.A., Sheremet Ya.A., Blume Ya.B., Yemets A.I. Arabidopsis root morphology and its correlation with cortical microtubule rearrangements caused by specific inhibition of protein kinases CK1, Tsitol Genet., 2019, vol. 53, no. 6, pp. 3-14.

In "Cytology and Genetics":
P. A. Karpov, Ya. A. Sheremet, Ya. B. Blume, A. I. Yemets Studying the Role of Protein Kinases CK1 in Organization of Cortical Microtubules in Arabidopsis thaliana Root Cells, Cytol Genet., 2019, vol. 53, no. 6, pp. 441450
DOI: 10.3103/S0095452719060033


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