Cytology and Genetics 2011,
vol. 45,
no. 1, 7-12,
doi: https://www.doi.org/10.3103/S0095452711010038
The conditions of high efficient chicory transformation with Mycobacterium tuberculosis antigene Đ•SAT6 have been determined. Transformation frequency was up to 86 % when the cotyledons were cultivated within 3 days without cefotaxime and then 1 day without kanamycine. DNA PCR-analysis has shown the presence both of selective nptII and target esxA genes in all analysed plants. At the same time RT-PCR has shown the presence of nptII transcripts for eight analysed lines and esxA transcripts for only five analysed lines.
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