TSitologiya i Genetika 2011, vol. 45, no. 1, 11-17
Cytology and Genetics 2011, vol. 45, no. 1, 7-12, doi: https://www.doi.org/10.3103/S0095452711010038

Effective agrobacterium-mediated transformation of chicory (Cichorium intybus L.) with Mycobacterium tuberculosis antigene ESAT6

Matvieieva N.A., Vasylenko M.Y., Shahovsky A.M., Bannykova M.O., Kvasko O.Y., Kuchuk N.V.

The conditions of high efficient chicory transformation with Mycobacterium tuberculosis antigene Đ•SAT6 have been determined. Transformation frequency was up to 86 % when the cotyledons were cultivated within 3 days without cefotaxime and then 1 day without kanamycine. DNA PCR-analysis has shown the presence both of selective nptII and target esxA genes in all analysed plants. At the same time RT-PCR has shown the presence of nptII transcripts for eight analysed lines and esxA transcripts for only five analysed lines.

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TSitologiya i Genetika
2011, vol. 45, no. 1, 11-17

Current Issue
Cytology and Genetics
2011, vol. 45, no. 1, 7-12,
doi: 10.3103/S0095452711010038

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