Identification of allelic state of leaf rust-resistance Lr34 gene in the cultivars of soft winter wheat of ukrainian breeding

The distribution of alleles at the Lr34 locus associated with leaf rust resistance among winter common wheat (Triticum aestivum L.) cultivars of Ukrainian breeding was studied. Co-dominant molecular-genetic marker cssfr5 was used for detection of the allelic condition of the Lr34 locus. The cultivars with detected allele Lr34(+) were identified as potentially «resistant» and the cultivars in which detected allele Lr34(–) were identified as potentially «susceptible». The collection of the cultivars (81 ones), created in the main plant breeding centers of Ukraine was analyzed. The allele Lr34(+) was identified in 44 % cultivars. Results were compared with the data about the distribution of the Lr34(+) in cultivars created in different countries.

Keywords: wheat, leaf rust (Puccinia triticina), leaf rust resistance, loci Lr34, polymerase chain reaction (PCR)