Morphological and immunophenotypical properties of human adult adipose tissue stromal cells (ATSC) at cultivation passage 0 and 4 as well as their ability to induced in vitro differentiation into adipogenic and osteogenic directions were studied in this work. It was shown that primary cultures of ATSC were characterized by the presence of the lower number of cells expressing mesenchymal markers (CD73, CD105) than the cells of the 4th passage, but contained endothelial progenitor cells expressing CD34 and capable to form capillary-like structures within extracellular matrix. Both cell populations could equally differentiate into adipogenic and osteogenic lineages.
Keywords: adipose tissue stromal cells, subculturing, differentiation, immunophenotype, endothelial progenitors
