Human nerve stem cells in vitro

Tsymbaluk V.I., Vasilyeva I.G., Olexenko N.P., Chopic N.G., Tsybko O.I., Galanta E.S., Snitcar N.D.

The expansion of Nest-positive nerve stem cells fraction in the culture of human fetal brain was investigated in this work. Our results demonstrated the valuable increase of the percentage of Nest-positive cells from 44.1 to 62.5 % of cell population at the response to the presence of EGF and bFGF and using the heat-inactivated serum in the culture medium. Further differentiation in culture tended to decrease of Nest-positive cell amount. Stimulation of the neuroinduction with retinoic acid resulted in decrease of the percentage of these cells in the population to 14.1 %. This data allowed obtaining the nerve cell population more effective for using as a graft material in cell therapy.

Key words: nestin, stem cells, brain, nerve cells culture

Tsitologiya i Genetika 2012, vol. 46, no. 1, pp. 71-74

E-mail: N.Oleksenko gmail.com

Tsymbaluk V.I., Vasilyeva I.G., Olexenko N.P., Chopic N.G., Tsybko O.I., Galanta E.S., Snitcar N.D. Human nerve stem cells in vitro, Tsitol Genet., 2012, vol. 46, no. 1, pp. 71-74.