Characterization of Streptomyces globisporus 1912 lnd­cluster region containing lndY, lndYR, lndW2 and lndW genes

SUMMARY. Streptomyces globisporus 1912 lnd-cluster region which flanks structural lndZ5-lndZ6 genes contains four open reading frames lndW, lndW2, lndYR and lndY. The latter one encodes putative proteinase which can regulate landomycin production and morphogenesis like LndYR. However, results of lndY overexpression and gene knockout showed that lndY did not participate in regulation of landomycin production and morphogenesis of Streptomyces globisporus 1912. Using transcriptional fusion of promoter lndWр to catechol dioxygenase reporter gene xylE the temporal character of interaction of promoter lndWр and repressor LndYR was studied.

Keywords: Streptomyces globisporus 1912, landomycins, effector molecule, kinases